RANBP10 Antibody - BSA Free

Western Blot: RANBP10 Antibody [NBP1-77372] - Human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: RANBP10 Antibody - BSA Free [NBP1-77372] - Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 u/mL.

Immunohistochemistry: RANBP10 Antibody - BSA Free [NBP1-77372] - Immunohistochemistry of RANBP10 in human skeletal muscle tissue with RANBP10 antibody at 5 ug/mL.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RANBP10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RANBP10 Antibody - BSA Free (NBP1-77372) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10. The immunogen is located within amino acids 380 - 430 of RANBP10. Amino Acid Squence: VASTSKQNHSKYPA
• Predicted Species: Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANBP10
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  RANBP10 Peptide (NBP1-77372PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RANBP10 Antibody - BSA Free

• KIAA1464FLJ31165
• RAN binding protein 10
• ran-binding protein 10
• RanBP10

Background

The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANBP10
• Uniprot:
  • EAW83167(Human)