RANBP10 Antibody

Images

 
Western Blot: RANBP10 Antibody [NBP1-77372] - Human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: RANBP10 Antibody [NBP1-77372] - Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: RANBP10 Antibody [NBP1-77372] - Human skeletal muscle tissue with RANBP10 antibody at 5 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RANBP10 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10. The immunogen is located within amino acids 380 - 430 of RANBP10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP10
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
RANBP10 Peptide (NBP1-77372PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RANBP10 Antibody

  • KIAA1464FLJ31165
  • RAN binding protein 10
  • ran-binding protein 10
  • RanBP10

Background

The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RANBP10
Uniprot