OXTR Antibody

Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.

Flow Cytometry: OXTR Antibody [NBP2-76347] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Staining of paraffin embedded Human Ovary with antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: Flow, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OXTR Antibody Summary

• Immunogen: Peptide with sequence C-LRTTRQKHSRL corresponding to internal region according to NP_000907.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OXTR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 8 ug/mL
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA Detection limit 1:64000
• Application Notes: Western blot: Preliminary experiments showed bands at 55 kDa and 26 kDa in Human Ovary and Breast lysates. Both bands were successfully blocked by incubation with the immunizing peptide at antibody concentration 2 ug/mL.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OXTR Antibody

• OT-R
• OXTR
• oxytocin receptor

Background

The protein encoded by the Oxytocin Receptor gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OXTR