RANBP9 Antibody (4P10V6)

Western Blot: RANBP9 Antibody (4P10V6) [NBP3-15702] - Western blot analysis of extracts of various cell lines, using RANBP9 antibody (NBP3-15702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: RANBP9 Antibody (4P10V6) [NBP3-15702] - Immunohistochemistry of paraffin-embedded human brain using RANBP9 Rabbit mAb (NBP3-15702) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: RANBP9 Antibody (4P10V6) [NBP3-15702] - Immunohistochemistry of paraffin-embedded rat testis using RANBP9 Rabbit mAb (NBP3-15702) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 4P10V6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

RANBP9 Antibody (4P10V6) Summary

• Description: Novus Biologicals Rabbit RANBP9 Antibody (4P10V6) (NBP3-15702) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RANBP9 (Q96S59). SGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSALNEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RANBP9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RANBP9 Antibody (4P10V6)

• BPM90
• BPM-L
• RAN binding protein 9
• Ran Binding Protein in the Microtubule organizing center
• ran binding protein, centrosomal
• ran-binding protein 9
• Ran-binding protein M
• RanBP7
• RanBP9
• RanBPM
• RANBPMnovel centrosomal protein RanBPM

Background

Just as heat shock proteins function as chaperones for exposed hydrophobic patches, importins act as chaperones for exposed basic domains, and it is suggested that this represents a major and general cellular function of importins (1). RanBP9, a novel interacting protein, was localized within the centrosome throughout the cell cycle. Overexpression of RanBP9 produced multiple spots which were colocalized with gamma-tubulin and acted as ectopic microtubule nucleation sites, resulting in a reorganization of microtubule network (2). It has been shown that RanBP9 can induce GTP-Ras association and Erk phosphorylation and elevate serum response element-luciferase (SRE-LUC) expression, indicating that RanBP9 can activate the Ras-Erk-SRE pathway. Also RanBP9 stimulates Ras activation by recruiting Sos (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANBP9