Rad51C Antibody (2H11/6) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Pm, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2H11/6
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Rad51C Antibody (2H11/6) [DyLight 405] Summary

Immunogen
His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Localization
Nuclear
Specificity
Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD51C
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Rad51C (2H11/6) antibody is useful for Western Blot, where a band can be seen at ~40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). Use in IHC-P was reported in the scientific literature (PMID: 23512992).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, yeast, and primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Rad51C Antibody (2H11/6) [DyLight 405]

  • BROVCA3
  • DNA repair protein RAD51 homolog 3
  • FANCO
  • MGC104277
  • R51H3
  • RAD51 homolog C (S. cerevisiae)
  • RAD51 homolog C
  • RAD51L2RAD51 (S. cerevisiae) homolog C
  • RAD51-like protein 2
  • yeast RAD51 homolog 3

Background

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51C Antibody (NB100-177V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-177APC
Biotin Labeled NB100-177B
DyLight 350 Labeled NB100-177UV
DyLight 488 Labeled NB100-177G
DyLight 550 Labeled NB100-177R
DyLight 650 Labeled NB100-177C
DyLight 680 Labeled NB100-177FR
DyLight 755 Labeled NB100-177IR
FITC Labeled NB100-177F
HRP Labeled NB100-177H
PE Labeled NB100-177PE

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Bioinformatics

Gene Symbol RAD51C