Rad51C Antibody (2H11/6) [DyLight 488]

• Reactivity: Hu, Mu, Pm, Ye
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: 2H11/6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Rad51C Antibody (2H11/6) [DyLight 488] Summary

• Immunogen: His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
• Localization: Nuclear
• Specificity: Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD51C
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This Rad51C (2H11/6) antibody is useful for Western Blot, where a band can be seen at ~40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). Use in IHC-P was reported in the scientific literature (PMID: 23512992).
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Rad51C Antibody (2H11/6) [DyLight 488]

• BROVCA3
• DNA repair protein RAD51 homolog 3
• FANCO
• MGC104277
• R51H3
• RAD51 homolog C (S. cerevisiae)
• RAD51 homolog C
• RAD51L2RAD51 (S. cerevisiae) homolog C
• RAD51-like protein 2
• yeast RAD51 homolog 3

Background

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD51C