DMC1 Antibody (1D12/4)

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Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clone
1D12/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DMC1 Antibody (1D12/4) Summary

Immunogen
This DMC1 Antibody (1D12/4) is made against His-tagged human DMC1, overexpressed in E. coli [UniProt# Q14565].
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DMC1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunoprecipitation
Application Notes
This DMC1 (1D12/4) antibody is useful for Western Blot, where a band can be seen at ~37 kDa for endogenous protein and ~40.5 kDa for recombinant his-tagged DMC1. Immunoprecipitation was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Testis Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 1
using
NB100-181 in the following applications:

Publications
Read Publication using
NB100-181 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse. Does not cross-react with human Rad51 in Western blot analysis.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for DMC1 Antibody (1D12/4)

  • disrupted meiotic cDNA1, yeast, homolog of
  • DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast)
  • DMC1 homologue
  • HsLim15
  • LIM15yeast homolog) meiosis-specific homologousrecombination
  • meiotic recombination protein DMC1/LIM15 homolog
  • MGC150472
  • MGC150473

Background

DMC1 (disrupted meiotic cDNA or LIM15) is a meiosis-specific gene that encodes a nuclear protein which is essential for meiotic homologous recombination, specifically in homologous strand assimilation, and MDC1 also participates in formation of synaptonemal complex as well as double-strand breaks repair at recombination hotspots. DMC1 is a member of RecA family and in eukaryotic cells; there are two RecA homologs, RAD51 and DMC1, which promote the search for homologous DNA sequences and DNA strand exchange resulting in the formation of joint molecules. RAD51 acts both in meiotic recombinations as well as during DNA repair in mitotically dividing cells, however, DMC1 is expressed only during meiosis only. DMC1 interacts with the MND1-PSMC3IP heterodimer and with BRCA2. Dmc1-/- mice are infertile due to decreased germ cells, failing to form synaptonemal complexes and arresting at the leptotene or zygotene stage in late meiosis I. In female mice lacking Dmc1, normal oogenesis is aborted in embryos, and germ cells are depleted in the adult ovary. The phenotypes of Dmc1-/- ovaries are similar to clinical characteristics found in women with premature ovarian failure (POF), however, mutations in the coding sequence of DMC1 were shown to not to associate with POF in women.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DMC1 Antibody (NB100-181)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Review for DMC1 Antibody (NB100-181) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-181:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence DMC1 NB100-181
Enlarge
1
reviewed by:
Sumit sandhu
IF Mouse 06/29/2020
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse spermatocytes
SpeciesMouse
LotB1

Comments

Comments*Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DMC1 Antibody (NB100-181) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on DMC1.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

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Recent Reviews

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1
1

Sumit sandhu
06/29/2020
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol DMC1
Entrez
Uniprot