Rad17 Antibody

Western Blot: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Detection by chemiluminescence.

Immunoprecipitation: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. For blotting immunoprecipitated Rad17, antibody was used at 1:100. Detection by chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Pm-Or
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Rad17 Antibody Summary

• Description: Novus Biologicals Goat Rad17 Antibody (NBP2-75345) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 564 and 614
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAD17
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 40 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4°C.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Rad17 Antibody

• CCYC
• cell cycle checkpoint protein (RAD17)
• cell cycle checkpoint protein RAD17
• FLJ41520
• HRAD17
• R24L
• RAD1 (S. pombe) homolog
• RAD1 homolog
• RAD17 homolog (S. pombe)
• Rad17
• Rad17-like protein
• RAD17SP
• RAD24
• RF-C activator 1 homolog
• RF-C/activator 1 homolog

Background

Rad17 is a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75943].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD17