Rad1 Antibody

Western Blot: Rad1 Antibody [NB100-56320] - Analysis of Rad1 in HEK293 cell lysate using anti-Rad1 antibody. Image from verified customer review.

Western Blot: Rad1 Antibody [NB100-56320] - Western blot analysis of Rad1 in A) HeLa and B) HEK293 cell lysate using this antibody at 4 ug/ml.

Product Discontinued

Rad1 Antibody Summary

• Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acid 133-148 of human Rad1 (accession number O60671), which is a homolog of Schizosaccharomyces pombe Rad1.
• Predicted Species: Mouse (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5 ug/ml
• Control:

  293 Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Rad1 Antibody

• cell cycle checkpoint protein Hrad1
• checkpoint control protein HRAD1
• DNA repair exonuclease rad1 homolog
• DNA repair exonuclease REC1
• EC 3.1.11.2
• exonuclease homolog RAD1
• HRAD1
• RAD1 (S. pombe) homolog
• RAD1 homolog (S. pombe)
• Rad1-like DNA damage checkpoint protein
• REC1cell cycle checkpoint protein RAD1

Background

The structure and integrity of DNA molecules can be spontaneously affected through intrinsic instability of chemical bonds in DNA or can be induced by chemical compounds and irradiation. DNA lesions are of many different types including single and double-strand breaks, inter and intra-strand cross-links and different kinds of base modification. DNA lesions hamper processes like transcription and replication resulting in cell cycle arrest, apoptosis and genomic instability (mutagenesis). DNA lesions have been implicated in many distinct genetically inherited diseases, in carcinogenesis, origin of genetic disorders and in aging. Rad1 is an essential component of cell cycle checkpoints activated by DNA damage and incomplete DNA replication. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage checkpoint response in S. pombe. Human HRAD1 gene is located on chromosome 5p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad1 Antibody (NB100-56320)(1)

Publication using NB100-56320

• Haynes B, Gajan A, Nangia-Makker P et al. RAD6B is a major mediator of triple negative breast cancer cisplatin resistance: Regulation of translesion synthesis/Fanconi anemia crosstalk and BRCA1 independence Biochim Biophys Acta Mol Basis Dis 2019-10-19 [PMID: 31639439] (WB, Human)

Review for Rad1 Antibody (NB100-56320) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Shilpa Agarwal WB Human 11/21/2014

Summary

• Application: Western Blot
• Sample Tested: Lysate from HEK293 cells
• Species: Human

Bioinformatics

• Gene Symbol: RAD1
• Entrez:
  • 5810(Human)
  • 19355(Mouse)
• Uniprot:
  • O60671(Human)