Rad1 Antibody

Images

 
Western Blot: Rad1 Antibody [NB100-56320] - analysis of Rad1 in HEK293 cell lysate using anti-Rad1 antibody. Image from verified customer review.
Western Blot: Rad1 Antibody [NB100-56320] - Western blot analysis of Rad1 in A) HeLa and B) HEK293 cell lysate using Rad1 antibody at 4 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Rad1 Antibody Summary

Immunogen
The antibody was raised against a synthetic peptide corresponding to the amino acid 133-148 of human Rad1 (accession number O60671), which is a homolog of Schizosaccharomyces pombe Rad1.
Predicted Species
Mouse (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
Positive Control
293 Lysate (NB800-PC6)
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56320 in the following application:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Rad1 Antibody

  • cell cycle checkpoint protein Hrad1
  • checkpoint control protein HRAD1
  • DNA repair exonuclease rad1 homolog
  • DNA repair exonuclease REC1
  • EC 3.1.11.2
  • exonuclease homolog RAD1
  • HRAD1
  • RAD1 (S. pombe) homolog
  • RAD1 homolog (S. pombe)
  • Rad1-like DNA damage checkpoint protein
  • REC1cell cycle checkpoint protein RAD1

Background

The structure and integrity of DNA molecules can be spontaneously affected through intrinsic instability of chemical bonds in DNA or can be induced by chemical compounds and irradiation. DNA lesions are of many different types including single and double-strand breaks, inter and intra-strand cross-links and different kinds of base modification. DNA lesions hamper processes like transcription and replication resulting in cell cycle arrest, apoptosis and genomic instability (mutagenesis). DNA lesions have been implicated in many distinct genetically inherited diseases, in carcinogenesis, origin of genetic disorders and in aging. Rad1 is an essential component of cell cycle checkpoints activated by DNA damage and incomplete DNA replication. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage checkpoint response in S. pombe. Human HRAD1 gene is located on chromosome 5p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Rad1 Antibody (NB100-56320) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56320:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Rad1 NB100-56320
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reviewed by:
Shilpa Agarwal
WB Human 11/21/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from HEK293 cells
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary Descriptionanti rabbit HRP-conjugated antibody
Secondary Manufacturer Cat#Life Technologies # A16066
Secondary Concentration1:10,000

Details

Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 20 sec exposure using X-ray film

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rad1 Antibody (NB100-56320) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Rad1 Antibody (NB100-56320)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Shilpa Agarwal
11/21/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD1
Entrez
Uniprot