Rab13 Antibody

Knockdown Validated: Rab13 Antibody [NBP1-85799] - Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136) licensed under a CC-BY license.

Knockdown Validated: Rab13 Antibody [NBP1-85799] - Cells treated with the indicated PMOs or siRNAs were analyzed by Western blot (upper panels) to detect RAB13 and GAPDH protein levels. Migration speed was assessed as in (B) from n = 55-78 cells (bottom graph). Bars: mean +/- s.e.m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136) licensed under a CC-BY license.

Western Blot: Rab13 Antibody [NBP1-85799] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB13 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Rab13 Antibody [NBP1-85799] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.

Western Blot: Rab13 Antibody [NBP1-85799] - Analysis in human cell line MCF-7.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human prostate shows weak membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Func, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rab13 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCS
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: Use in Proximity Ligation Assay reported in scientific literature (PMID:31290739).For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Rab13 Protein (NBP1-85799PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:31290739).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rab13 Antibody

• Cell growth-inhibiting gene 4 protein
• growth-inhibiting gene 4 protein
• Rab13
• RAB13, member RAS oncogene family
• RAS-associated protein RAB13
• ras-related protein Rab-13

Background

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab13 Antibody (NBP1-85799)(5)

Publications using NBP1-85799

• Eaton A Effects of Stretch on the Bladder Umbrella Cell Apical Junctional Ring Thesis 2020-01-01 (WB, Rat)
• Moissoglu K, Stueland M, Gasparski AN et al. RNA localization and co-translational interactions control RAB13 GTPase function and cell migration EMBO J. 2020-09-18 [PMID: 32946136] (ICC/IF, WB, Human)
• Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport Moissoglu K, Yasuda K, Wang T et al. Elife [PMID: 31290739] (PLA, WB, Mouse)
• Eaton AF, Clayton DR, Ruiz WG et al. Expansion and Contraction of the Umbrella Cell Apical Junctional Ring in Response to Bladder Filling and Voiding Mol. Biol. Cell 2019-06-05 [PMID: 31166831]
• Abou-Zeid N, Pandjaitan R, Sengmanivong L et al. MICAL-like1 mediates epidermal growth factor receptor endocytosis. Mol Biol Cell 2011-09-01 [PMID: 21795389]

Bioinformatics

• Gene Symbol: RAB13