Recombinant Human RAB10 His Protein

SDS-Page: Recombinant Human RAB10 Protein [NBP2-23392]

• Reactivity: Hu
• Applications: WB, PAGE
• Concentration: 0.5 mg/ml

Recombinant Human RAB10 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-200 of Human RAB10
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RAB10
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. Use in WB reported in scientific literature (PMID:10.1242/dmm.048017)
• Theoretical MW: 24.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human RAB10 His Protein

• GTP-binding protein RAB10
• RAB10, member RAS oncogene family
• ras-related GTP-binding protein
• ras-related protein Rab-10

Background

RAB10 is a member of the Ras superfamily of small GTP-binding proteins. This protein is a key regulator of endocytic recycling, especially in the basolateral recycling pathways of polarized epithelial cells. It is required for the recruitment of CNT-1 to endosomal membranes in the intestinal epithelium. Recombinant human RAB10 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for RAB10 Recombinant Protein (NBP2-23392)(3)

Publications using NBP2-23392

• Yuan Yuan, Huizhong Li, Kashyap Sreeram, Tuyana Malankhanova, Ravindra Boddu, Samuel Strader, Allison Chang, Nicole Bryant, Talene A. Yacoubian, David G. Standaert, Madalynn Erb, Darren J. Moore, Laurie H. Sanders, Michael W. Lutz, Dmitry Velmeshev, Andrew B. West Single molecule array measures of LRRK2 kinase activity in serum link Parkinson’s disease severity to peripheral inflammation Molecular Neurodegeneration 2024-06-11 [PMID: 38862989]
• Miyamoto T, Hosoba K, Itabashi T et al. Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome EMBO J. 2020-05-05 [PMID: 32368833] (In vitro)

Bioinformatics

• Gene Symbol: RAB10