QKI/Quaking Antibody - BSA Free

Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

QKI/Quaking Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit QKI/Quaking Antibody - BSA Free (NB300-240) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-QKI/Quaking Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to the C-terminus of isoform 5 of human quaking homolog according to entry AAH19917.1 (GeneID 9444). The epitope is not found in isoforms 1, 2, 3, 4, 6, 7 or 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU7, Q969L9 and Q96PU6, respectively.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: QKI
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2 - 10 mcg/mg
  • Western Blot 1:5000-1:25000
• Application Notes: By Western Blot, this antibody recognizes a protein ~38 kDa, representing QKI. Immunoprecipitation was reported in scientific literature. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Nuclear Cell Lysate
  QKI/Quaking Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for QKI/Quaking Antibody - BSA Free

• DKFZp586I0923
• HKQ
• homolog of mouse quaking QKI (KH domain RNA binding protein)
• Hqk
• hqkI
• protein quaking
• QK
• QK1
• QK3
• quaking homolog, KH domain RNA binding (mouse)
• RNA binding protein HQK

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for QKI/Quaking Antibody (NB300-240)(2)

Publications using NB300-240

• Barry G, Briggs JA, Vanichkina DP et al. The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Mol Psychiatry 2013-04-30 [PMID: 23628989] (IP, Human)
• Novikov L, Park JW, Chen H et al. QKI-mediated alternative splicing of the histone variant MacroH2A1 regulates cancer cell proliferation. Mol Cell Biol 2011-10-01 [PMID: 21844227]

Bioinformatics

• Gene Symbol: QKI
• Entrez:
  • 9444(Human)
• Uniprot:
  • AAH19917.1(Human)