Cathepsin C/DPPI Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) ...read more
Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. ...read more
Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 35 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Cathepsin ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Cathepsin C/DPPI Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin C/DPPI
Asp25-Leu462
Accession # P97821
Specificity
Detects mouse Cathepsin C/DPPI in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) Cathepsin C is observed and less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin B, rmCathepsin D, rmCathepsin H, and rmCathepsin X/Z/P is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSC
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-1 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1034 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin C/DPPI Antibody [Unconjugated]

  • Cathepsin C
  • cathepsin CEC 3.4.14.1
  • Cathepsin J
  • CPPIHMS
  • CTSC
  • dipeptidyl peptidase 1
  • Dipeptidyl peptidase I
  • Dipeptidyl transferase
  • dipeptidyl-peptidase I
  • DPP1
  • DPPI
  • DPP-I
  • JP
  • JPD
  • PALS
  • PLS

Background

Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).

  1. Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 1192, Academic Press, San Diego.
  2. Dahl, S.W. et al. (2001) Biochemistry 40:1671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin C/DPPI Antibody (AF1034)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-OCT embedded, WB.


Filter By Application
ICC
(2)
IHC
(1)
IHC-OCT embedded
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1034 Applications Species
T Shimizu, W Wisessmith, J Li, M Abe, K Sakimura, B Chetsawang, Y Sahara, K Tohyama, KF Tanaka, K Ikenaka The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model Glia, 2017;0(0):. 2017 [PMID: 28251676] (ICC,, Mouse) ICC Mouse
Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015;10(4):e0120348. 2015 [PMID: 25927437] (IHC-OCT embedded,, Mouse) IHC-OCT embedded Mouse
Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015;14(0):39. 2015 [PMID: 25744631] (WB,, Mouse) WB Mouse
Muller S, Faulhaber A, Sieber C, Pfeifer D, Hochberg T, Gansz M, Deshmukh S, Dauth S, Brix K, Saftig P, Peters C, Henneke P, Reinheckel T The endolysosomal cysteine cathepsins L and K are involved in macrophage-mediated clearance of Staphylococcus aureus and the concomitant cytokine induction. FASEB J, 2014;28(1):162-75. 2014 [PMID: 24036885] (WB,, Mouse) WB Mouse
Fan K, Wu X, Fan B, Li N, Lin Y, Yao Y, Ma J Up-regulation of microglial cathepsin C expression and activity in lipopolysaccharide-induced neuroinflammation. J Neuroinflammation, 2012;9(1):96. 2012 [PMID: 22607609] (IHC,, Human) IHC Human
Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011;25(23):2465-79. 2011 [PMID: 22156207] (WB,, Mouse) WB Mouse
Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice. PLoS ONE, 2011;6(10):e26247. 2011 [PMID: 22022579] (WB,, Mouse) WB Mouse
Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin. Blood, 2011;118(12):3384-91. 2011 [PMID: 21795745] (ICC,, Mouse) ICC Mouse
Melo FR, Waern I, Ronnberg E, Abrink M, Lee DM, Schlenner SM, Feyerabend TB, Rodewald HR, Turk B, Wernersson S, Pejler G A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway. J. Biol. Chem., 2011;286(7):5423-33. 2011 [PMID: 21123167] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol CTSC
Uniprot