Pyridoxal Kinase/PDXK Antibody

Images

 
Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Staining of human gall bladder shows moderate cytoplasmic and nuclear positivity in glandular cells.
Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pyridoxal Kinase/PDXK Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:PNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDXK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunofluorescence
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Pyridoxal Kinase/PDXK Protein (NBP1-88283PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (86%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pyridoxal Kinase/PDXK Antibody

  • C21orf124
  • C21orf97
  • chromosome 21 open reading frame 124
  • DKFZp566A071
  • EC 2.7.1.35
  • FLJ21324
  • FLJ37311
  • HEL-S-1a
  • MGC15873
  • MGC31754
  • MGC52346
  • PDXK
  • PKH
  • PKHchromosome 21 open reading frame 97
  • PNK
  • PNKhuman pyridoxal kinase, EC 2.7.1.3510FLJ31940
  • PRED79
  • pyridoxal (pyridoxine, vitamin B6) kinase
  • Pyridoxal Kinase
  • pyridoxamine kinase
  • Pyridoxine kinase
  • Vitamin B6 Kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDXK
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