PNPO Antibody (OTI1G9)

Western Blot: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO .

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Adenocarcinoma of Human colon tissue.

Flow Cytometry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Jurkat cells, using PNPO antibody,(Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human pancreas tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human prostate tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human liver tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human breast tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human lymphoma tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Carcinoma of Human prostate tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Carcinoma of Human liver tissue.

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Human Kidney tissue.

Flow Cytometry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Hela cells, using PNPO antibody (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PNPO Antibody (OTI1G9) Summary

• Description: Novus Biologicals Mouse PNPO Antibody (OTI1G9) (NBP2-45361) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PNPO Antibody (OTI1G9)

• EC 1.4.3.5
• FLJ10535
• PDXPO
• pyridoxal 5'-phosphate synthase
• pyridoxamine 5'-phosphate oxidase
• Pyridoxamine-phosphate oxidase
• pyridoxine 5'-phosphate oxidase
• pyridoxine-5'-phosphate oxidase

Background

Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNPO