Pygopus-1 Antibody

Western Blot: Pygopus-1 Antibody [NBP1-42666] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Pygo1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody NBP1-42665, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Pygopus-1 Antibody Summary

• Description: Novus Biologicals Rabbit Pygopus-1 Antibody (NBP1-42666) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 369 and 419 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYGO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pygopus-1 Antibody

• DKFZp547G0910
• Pygo1
• pygopus 1
• pygopus homolog 1 (Drosophila)
• pygopus homolog 1
• Pygopus1
• Pygopus-1
• pygopus-like protein 1

Background

PYGO1, also known as Pygopus homolog 1, is a 45kDa 419 amino acid protein. PYGO1 is involved in signal transduction through the Wnt pathway. Current research on PYGO1 is being conducted in relation to acute diarrhea and colorectal cancer. PYGO1 is linked to the WTN signaling pathway where it interacts with BCL9, LENG8, CTNNB1, SPTBN1 and GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYGO1
• Entrez:
  • 26108(Human)