Purine Nucleoside Phosphorylase/PNP Antibody - BSA Free

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Purine Nucleoside Phosphorylase/PNP Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Purine Nucleoside Phosphorylase/PNP. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PNP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (88%), Mouse (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Purine Nucleoside Phosphorylase/PNP Antibody - BSA Free

• EC 2.4.2.1
• FLJ94043
• FLJ97288
• Inosine phosphorylase
• MGC117396
• MGC125915
• MGC125916
• NPFLJ97312
• nucleoside phosphorylase
• PNP
• PRO1837
• PUNP
• purine nucleoside phosphorylase
• purine-nucleoside:orthophosphate ribosyltransferase

Background

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Purine Nucleoside Phosphorylase/PNP Antibody (NBP2-19603)(1)

Publication using NBP2-19603

• Zhao L, Zhang C, Luo X et al. CD36 palmitoylation disrupts free fatty acid metabolism and promotes tissue inflammation in non-alcoholic steatohepatitis Journal of Hepatology 2018-04-01 [PMID: 29705240] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: PNP
• Uniprot:
  • P00491()