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DCK Antibody (10412)

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Western Blot: DCK Antibody (10412) [NBP2-43639] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (10412) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: DCK Antibody (10412) [NBP2-43639] - DCK antibody (10412) detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at ...read more
Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human lung cancer. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at ...read more
Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human colon carcinoma. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: DCK Antibody (10412) [NBP2-43639] - Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 ug of DCK antibody (10412) or DCK antibody (NBP2-16108). Western blot analysis was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
10412
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DCK Antibody (10412) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DCK
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-43639 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for DCK Antibody (10412)

  • DCK
  • deoxycytidine kinase
  • EC 2.7.1
  • EC 2.7.1.113
  • EC 2.7.1.74
  • EC 2.7.1.76
  • MGC117410
  • MGC138632

Background

DCK (Deoxycytidine kinase) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DCK Antibody (NBP2-43639) (0)

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Review for DCK Antibody (NBP2-43639) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-43639:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot DCK NBP2-43639
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4
reviewed by:
Masashi Okada
WB Human 09/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
Lot41967

Comments

Comments3 tumor cell lines (10ug lysate) are loaded.
1/2000 dil in 2% BSA/TBST, incubated for o/n at 4C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DCK Antibody (NBP2-43639) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Masashi Okada
09/14/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol DCK