Purine Nucleoside Phosphorylase/PNP Antibody [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-231 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, LNCaP human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 36 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Purine Nucleoside Phosphorylase/PNP Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Purine Nucleoside Phosphorylase/PNP
  Met1-Ser289
  Accession # P00491
• Specificity: Detects human Purine Nucleoside Phosphorylase/PNP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PNP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Purine Nucleoside Phosphorylase/PNP Antibody [Unconjugated]

• EC 2.4.2.1
• FLJ94043
• FLJ97288
• Inosine phosphorylase
• MGC117396
• MGC125915
• MGC125916
• NPFLJ97312
• nucleoside phosphorylase
• PNP
• PRO1837
• PUNP
• purine nucleoside phosphorylase
• purine-nucleoside:orthophosphate ribosyltransferase

Background

Purine Nucleoside Phosphorylase (PNP) catalyzes the phophorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. Physiological substrates of PNP include inosine, guanosine, and 2'-deoxyguanosine, but not adenosine (1). PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T‑lymphocyte function and neurologic dysfunction (2, 3). PNP is used in assays for the measurement of inorganic phosphate (4).

1. Schramm, V.L. (1998) Annu. Rev. Biochem. 67:693.
2. Stoop, W. et al. (1977) N. Eng. J. Med. 296:651.
3. Markert, M.L. (1991) Immunodefic. Rev. 3:45.
4. Webb, M.R. (1992) Proc. Natl. Acad. Sci. USA. 89:4884.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNP
• Uniprot:
  • P00491()