PUM2 Antibody - BSA Free

Western Blot: PUM2 Antibody [NBP2-20009] - Sample (20 ug of whole cell lysate) A: Human ESC 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-20009] - Pumilio 2 antibody detects Pumilio 2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pumilio 2 stained by Pumilio 2 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: PUM2 Antibody [NBP2-20009] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term diluted at 1:500.

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term (NBP2-20009) diluted at 1:500.

Pumilio 2 antibody [C2C3], C-term detects Pumilio 2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pumilio 2 stained by Pumilio 2 antibody [C2C3], C-term (NBP2-20009) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ch, Fi
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PUM2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PUM2 Antibody - BSA Free (NBP2-20009) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PUM2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (100%), Chicken (100%), Fish (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PUM2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Japanese Medaka (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PUM2 Antibody - BSA Free

• FLJ36528
• KIAA0235PUMH2PUML2
• MGC138251
• MGC138253
• PUM2
• PUMH2
• pumilio (Drosphila) homolog 2
• pumilio homolog 2 (Drosophila)
• pumilio homolog 2
• Pumilio-2
• PUML2

Background

Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PUM2
• Uniprot:
  • Q8TB72()