PUM1 Antibody - BSA Free

Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.

Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu, Pm-Or
• Applications: WB, GS, ICC/IF, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PUM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat PUM1 Antibody - BSA Free (NB100-259) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-PUM1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PUM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Gel Super Shift Assays and Immunoprecipitation were reported in scientific literature (PMID: 23071749). Use in ICC/IF was reported in scientific literature (PMID: 23071749).
• Theoretical MW: 126 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PUM1 Antibody - BSA Free

• HSPUM
• KIAA0099PUML1
• PUM1
• PUMH
• PUMH1
• PUMH1pumilio homolog 1
• pumilio (Drosophila) homolog 1
• pumilio homolog 1 (Drosophila)
• Pumilio-1
• PUML1

Background

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PUM1 Antibody (NB100-259)(7)

Publications using NB100-259

• Takei N, Takada Y, Kawamura S et al. Changes in subcellular structures and states of pumilio 1 regulate the translation of target Mad2 and cyclin B1 mRNAs Journal of Cell Science 2020-05-19 [PMID: 33148609]
• Zhu X, Wang Y, Pi W et al. NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human gamma-globin gene. PLoS One. 2012-10-10 [PMID: 23071749] (GS, IP, Human)
• Ma L, Dong F, Zaid M et al. ABCA1 protein enhances Toll-like receptor 4 (TLR4)-stimulated interleukin-10 (IL-10) secretion through protein kinase A (PKA) activation. J Biol Chem 2012-11-23 [PMID: 23055522]
• Vessey JP, Schoderboeck L, Gingl E et al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 2010-02-01 [PMID: 20133610]
• Morris AR, Mukherjee N, Keene JD et al. Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets. Mol Cell Biol 2008-06-01 [PMID: 18411299]
• Chiu YL, Witkowska HE, Hall SC et al. High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition. Proc Natl Acad Sci U S A 2006-10-01 [PMID: 17030807]
• Uyhazi KE, Yang Y, Liu N et al. Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of PostTranscriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis bioRxiv 2019-08-30

Review for PUM1 Antibody (NB100-259) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Katherine Uyhazi WB Human 07/22/2010

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate, Sample Amount: 20ng
• Species: Human
• Lot: 1A

Bioinformatics

• Gene Symbol: PUM1
• Entrez:
  • 9698(Human)
  • 80912(Mouse)
• Uniprot:
  • Q14671(Human)