PUM1 Antibody

Images

 
Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ...read more
Western Blot: PUM1 Antibody [NB100-259] - NIH-3T3 cell lysate (50 ug loaded), probed with Pumilio-1 antibody diluted at 0.1 ug/ml.
Western Blot: PUM1 Antibody [NB100-259] - Analysis of human Pumilio 1, using NB100-259. Sample: RIPA extract (10 ug) from HeLa cells (CIP, calf intestinal phosphatase).
Biological Strategies: Western Blot: PUM1 Antibody [NB100-259] - Detection of Human and Mouse Pumillio 1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 ...read more
Independent Antibodies: Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, GS, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PUM1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PUM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1-10 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes bands around 150 kDa representing Pumilio. Gel Super Shift Assays and Immunoprecipitation were reported in scientific literature. Use in ICC/IF was reported in scientific literature (PMID: 23071749).
Theoretical MW
126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-259 in the following applications:

Publications
Read Publications using
NB100-259 in the following applications:

  • GS
    1 publication
  • IP
    1 publication

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PUM1 Antibody

  • HSPUM
  • KIAA0099PUML1
  • PUM1
  • PUMH
  • PUMH1
  • PUMH1pumilio homolog 1
  • pumilio (Drosophila) homolog 1
  • pumilio homolog 1 (Drosophila)
  • Pumilio-1
  • PUML1

Background

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PUM1 Antibody (NB100-259)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: GS, IP.


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GS
(1)
IP
(1)
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Human
(1)
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Review for PUM1 Antibody (NB100-259) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-259:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Katherine Uyhazi
WB Human 07/22/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, Sample Amount: 20ng
SpeciesHuman
Lot1A
CommentsVery clean -- 1 or 2 bands (looks like doublet on minimal exposure) slightly under 150 kD marker

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBS-T, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:10,000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Goat HRP, Secondary Ab Dilution Ratio: 1:10,000

Details

Detection NotesDetection Method: Film, Exposure Time: 5 minutes, Specific Bands: 140 kDa

Comments

CommentsVery clean -- 1 or 2 bands (looks like doublet on minimal exposure) slightly under 150 kD marker

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PUM1 Antibody (NB100-259) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on PUM1

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Katherine Uyhazi
07/22/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol PUM1
Entrez
Uniprot