VASA Antibody [Unconjugated]

Images

 
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG ...read more
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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VASA Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (AF2030)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 6 applications: Flow, ICC, IHC, IHC - Paraffin embedded, IHC Paraffin-embedded, IHC-P.


Filter By Application
Flow
(3)
ICC
(7)
IHC
(3)
IHC - Paraffin embedded
(1)
IHC Paraffin-embedded
(3)
IHC-P
(1)
All Applications
Filter By Species
Human
(13)
Mouse
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF2030 Applications Species
Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells Nat Commun, 2018;9(1):1873. 2018 [PMID: 29760424] (IHC,, Human) IHC Human
JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Stem Cell Res, 2018;27(0):46-56. 2018 [PMID: 29324254] (IHC-P,, Human) IHC-P Human
N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility Sci Rep, 2017;7(1):10011. 2017 [PMID: 28855574] (IHC,, Mouse) IHC Mouse
AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23 Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28579394] (ICC,, Primate - Macaca mulatta (Rhesus Macaque)) ICC Primate - Macaca mulatta (Rhesus Macaque)
C Eguizabal Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Stem Cells, 2016;0(0):. 2016 [PMID: 27300161] (IHC - Paraffin embedded,, Human) IHC - Paraffin embedded Human
Hu, Yueh-Chi, Nicholls, Peter K, Soh, Y Q Shir, Daniele, Joseph R, Junker, Jan Phil, van Oudenaarden, Alexande, Page, David C Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling. PLoS Genet, 2015;11(3):e1005019. 2015 [PMID: 25739037] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Heeren A, van Iperen L, Klootwijk D, de Melo Bernardo A, Roost M, Gomes Fernandes M, Louwe L, Hilders C, Helmerhorst F, van der Westerlaken L, Chuva de Sousa Lopes S Development of the follicular basement membrane during human gametogenesis and early folliculogenesis. BMC Dev Biol, 2015;15(0):4. 2015 [PMID: 25605128] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Dominguez, Antonia, Chiang, H Rosari, Sukhwani, Meena, Orwig, Kyle E, Reijo Pera, Renee A Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep, 2014;4(0):6432. 2014 [PMID: 25242416] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Lim J, Shim M, Lee J, Lee D Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells. PLoS ONE, 2014;9(4):e90454. 2014 [PMID: 24690677] (ICC,, Human) ICC Human
Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential. Stem Cells Dev, 2013;22(23):3141-55. 2013 [PMID: 23829223] (ICC,, Human) ICC Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol DDX4
Uniprot