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VASA Antibody [Unconjugated]

Images

 
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG ...read more
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with ...read more
Immuno-staining on control and PMSG treated ovarian sections.A-C: Immunostaining with anti-PCNA antibody. Note minimal PCNA staining in control OSE. Increased staining is observed after PMSG treatment in both the OSE ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

VASA Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDX4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (AF2030)(60)

We have publications tested in 5 confirmed species: Human, Mouse, Monkey, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse.

We have publications tested in 10 applications: Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(11)
ICC/IF
(1)
IF
(1)
IHC
(9)
IHC-Fr
(1)
IHC-P
(6)
Immunocytochemistry
(2)
Immunohistochemistry
(10)
Western Blot
(2)
All Applications
Filter By Species
Human
(19)
Mouse
(15)
Monkey
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using AF2030 Applications Species
Pathak, M;Spradling, AC; Mouse germline cysts contain a fusome-like structure that mediates oocyte development eLife 2026-03-11 [PMID: 41811096] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Atkins, GR;Hvasta-Gloria, RL;Ausavarungnirun, C;Pombar, CR;Hardy, JJ;Sukhwani, M;Barnard, EP;Pollock, N;Malizio, M;Sheng, Y;Brieño-Enríquez, MA;Castro, C;Chu, T;Yatsenko, AN;Orwig, KE; Tex11 Mutant Mouse Models of Human Azoospermia bioRxiv : the preprint server for biology 2026-02-18 [PMID: 41756962] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Ushuhuda, EG;Nguyen, JT;Pfaltzgraff, NG;Kofron, M;Mikedis, MM; MEIOC prevents continued mitotic cycling and promotes meiotic entry during mouse oogenesis bioRxiv : the preprint server for biology 2025-06-15 [PMID: 40661394] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Baskin, LS;Cao, M;Li, Y;Baker, L;Cooper, CS;Cunha, GR; Ovotesticular cords and ovotesticular follicles: New histologic markers for human ovotesticular syndrome Journal of pediatric urology 2024-01-06 [PMID: 38218629] (Immunohistochemistry, Human) Immunohistochemistry Human
Penny A. F. Whiley, Michael C. M. Luu, Liza O'Donnell, David J. Handelsman, Kate L. Loveland Testis exposure to unopposed/elevated activin A in utero affects somatic and germ cells and alters steroid levels mimicking phthalate exposure Front Endocrinol (Lausanne) 2023-09-01 [PMID: 37727456]
M. Bashawat, B.C. Braun, K. Müller, B.P. Hermann Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development – A model for wild felids Theriogenology Wild 2023-07-18 [PMID: 37461433]
Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764] (Immunocytochemistry, Human) Immunocytochemistry Human
Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N; Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells Cell reports 2023-07-04 [PMID: 37405912] (ICC/IF, Western Blot, IHC, Mouse) ICC/IF, Western Blot, IHC Mouse
AS Bailey, MT Fuller YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation bioRxiv : the preprint server for biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36747642] (IHC, Mouse) IHC Mouse
Yasunari Seita, Keren Cheng, John R McCarrey, Nomesh Yadu, Ian H Cheeseman, Alec Bagwell et al. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells eLife 2023-01-31 [PMID: 36719274]
Show All 60 Publications.

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Bioinformatics

Gene Symbol DDX4
Uniprot