VASA Antibody [Unconjugated]

Images

 
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG ...read more
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

VASA Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
NB100-62346
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP1-91011
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P
AF009
Species: Hu
Applications: WB, IHC
NB100-2437
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB600-749
Species: Hu, Mu, Rt, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-06062
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-37398
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NB300-232
Species: Hu, Mu, Rt, Po, Bv, Ch, Fe, Pa
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
H00388112-B01P
Species: Hu
Applications: WB
AF1997
Species: Hu
Applications: WB, ChIP, ICC
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
NBP2-45552
Species: Hu
Applications: WB, IHC

Publications for VASA Antibody (AF2030)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 6 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow Cytometry
(4)
ICC
(8)
IF
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(5)
All Applications
Filter By Species
Human
(15)
Mouse
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF2030 Applications Species
S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice PLoS ONE, 2020;15(1):e0227411. 2020 [PMID: 31910233] (IF, Mouse) IF Mouse
BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids Cell Rep, 2018;25(6):1650-1667.e8. 2018 [PMID: 30404016] (IHC-Fr, Human) IHC-Fr Human
JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance BMC Biol., 2018;16(1):104. 2018 [PMID: 30236109] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells Nat Commun, 2018;9(1):1873. 2018 [PMID: 29760424] (IHC, Human) IHC Human
A Bothun, Y Gao, Y Takai, O Ishihara, H Seki, B Karger, J Tilly, DC Woods Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis Stem Cells Dev., 2018;0(0):. 2018 [PMID: 29631484] (ICC, Human) ICC Human
JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Stem Cell Res, 2018;27(0):46-56. 2018 [PMID: 29324254] (IHC-P, Human) IHC-P Human
N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility Sci Rep, 2017;7(1):10011. 2017 [PMID: 28855574] (IHC, Mouse) IHC Mouse
AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23 Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28579394] (ICC, Primate - Macaca mulatta (Rhesus Macaque)) ICC Primate - Macaca mulatta (Rhesus Macaque)
C Eguizabal Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Stem Cells, 2016;0(0):. 2016 [PMID: 27300161] (IHC-P, Human) IHC-P Human
Hu, Yueh-Chi, Nicholls, Peter K, Soh, Y Q Shir, Daniele, Joseph R, Junker, Jan Phil, van Oudenaarden, Alexande, Page, David C Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling. PLoS Genet, 2015;11(3):e1005019. 2015 [PMID: 25739037] (IHC-P, Mouse) IHC-P Mouse
Show All 22 Publications.

Reviews for VASA Antibody (AF2030) (0)

There are no reviews for VASA Antibody (AF2030). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VASA Antibody (AF2030) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2030

Additional VASA Products

Bioinformatics Tool for VASA Antibody (AF2030)

Discover related pathways, diseases and genes to VASA Antibody (AF2030). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VASA Antibody (AF2030)

Discover more about diseases related to VASA Antibody (AF2030).
 

Pathways for VASA Antibody (AF2030)

View related products by pathway.

PTMs for VASA Antibody (AF2030)

Learn more about PTMs related to VASA Antibody (AF2030).

Blogs on VASA

There are no specific blogs for VASA, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VASA Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol DDX4
Uniprot