VASA Antibody [Unconjugated]

Images

 
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG ...read more
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VASA Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDX4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (AF2030)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 6 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow Cytometry
(4)
ICC
(8)
IF
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(5)
All Applications
Filter By Species
Human
(16)
Mouse
(7)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF2030 Applications Species
SP Windley, C Mayère, AE McGovern, NL Harvey, S Nef, Q Schwarz, S Kumar, D Wilhelm Loss of NEDD4 causes complete XY gonadal sex reversal in mice Cell Death & Disease, 2022;13(1):75. 2022 [PMID: 35075134] (IHC, Mouse) IHC Mouse
J Guo, E Sosa, T Chitiashvi, X Nie, EJ Rojas, E Oliver, DonorConne, K Plath, JM Hotaling, JB Stukenborg, AT Clark, BR Cairns Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33453151] (IHC, Human) IHC Human
S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice PLoS ONE, 2020;15(1):e0227411. 2020 [PMID: 31910233] (IF, Mouse) IF Mouse
BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids Cell Rep, 2018;25(6):1650-1667.e8. 2018 [PMID: 30404016] (IHC-Fr, Human) IHC-Fr Human
JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance BMC Biol., 2018;16(1):104. 2018 [PMID: 30236109] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells Nat Commun, 2018;9(1):1873. 2018 [PMID: 29760424] (IHC, Human) IHC Human
A Bothun, Y Gao, Y Takai, O Ishihara, H Seki, B Karger, J Tilly, DC Woods Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis Stem Cells Dev., 2018;0(0):. 2018 [PMID: 29631484] (ICC, Human) ICC Human
JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Stem Cell Res, 2018;27(0):46-56. 2018 [PMID: 29324254] (IHC-P, Human) IHC-P Human
N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility Sci Rep, 2017;7(1):10011. 2017 [PMID: 28855574] (IHC, Mouse) IHC Mouse
AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23 Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28579394] (ICC, Primate - Macaca mulatta (Rhesus Macaque)) ICC Primate - Macaca mulatta (Rhesus Macaque)
Show All 24 Publications.

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Bioinformatics

Gene Symbol DDX4
Uniprot