PUM2 Antibody

Images

 
Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal ...read more
Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PUM2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369).
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PUM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunoprecipitation 5-10 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes bands at ~135-160 kDa, representing Pum2. A non-specific band at ~40 kDa may be seen in mouse lysates and just above 250 kDa in human lysates.
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-387 in the following applications:

Publications
Read Publications using
NB100-387 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25097251).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PUM2 Antibody

  • FLJ36528
  • KIAA0235PUMH2PUML2
  • MGC138251
  • MGC138253
  • PUM2
  • PUMH2
  • pumilio (Drosphila) homolog 2
  • pumilio homolog 2 (Drosophila)
  • pumilio homolog 2
  • Pumilio-2
  • PUML2

Background

A component of conserved cellular machinery required for germ cell development, Pum2 is a human homolog of Pumilio, which helps maintain germ line stem cells in Drosophila and C. elegans. Pum2 forms a stable complex with DAZ through the same functional domain required for RNA binding and protein-protein interactions. Pum2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, IP

Publications for PUM2 Antibody (NB100-387)(5)

Review for PUM2 Antibody (NB100-387) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-387:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Katherine Uyhazi
WB Human 07/22/2010
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Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, Sample Amount: 20ug
SpeciesHuman
CommentsExcellent but with some minor background bands

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBS-T, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:8,000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Rabbit HRP, Secondary Ab Dilution Ratio: 1:10,000

Details

Detection NotesDetection Method: Film, Exposure Time: 5 minutes, Specific Bands: 130 kDa, Non-Specific Bands: 65, 35 kDa

Comments

CommentsExcellent but with some minor background bands

Product General Protocols

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Video Protocols

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FAQs for PUM2 Antibody (NB100-387) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for PUM2 Antibody (NB100-387)

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Blogs on PUM2

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Katherine Uyhazi
07/22/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol PUM2
Entrez
Uniprot