PUM2 Antibody (7M1B1)

Western Blot: PUM2 Antibody (7M1B1) [NBP3-15913] - Western blot analysis of extracts of Rat testis, using PUM2 antibody (NBP3-15913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Western Blot: PUM2 Antibody (7M1B1) [NBP3-15913] - Western blot analysis of extracts of various cell lines, using PUM2 antibody (NBP3-15913) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 7M1B1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PUM2 Antibody (7M1B1) Summary

• Description: Novus Biologicals Rabbit PUM2 Antibody (7M1B1) (NBP3-15913) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PUM2 (Q8TB72). MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQGFHGNSEVNAILSPRSESGGLGVSMVEYVLS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PUM2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PUM2 Antibody (7M1B1)

• FLJ36528
• KIAA0235PUMH2PUML2
• MGC138251
• MGC138253
• PUM2
• PUMH2
• pumilio (Drosphila) homolog 2
• pumilio homolog 2 (Drosophila)
• pumilio homolog 2
• Pumilio-2
• PUML2

Background

Pumilio 2 is a component of conserved cellular machinery required for germ cell development, Pum2 is a human homolog of Pumilio, which helps maintain germ line stem cells in Drosophila and C. elegans. Pum2 forms a stable complex with DAZ through the same functional domain required for RNA binding and protein-protein interactions. Pum2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUM2