PUM1 Antibodies
We offer PUM1 Antibodies for use in common research applications: ELISA, Gel Super Shift Assays, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each PUM1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PUM1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PUM1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PUM1 polyclonal antibodies and browse our PUM1 monoclonal antibody catalog.
Alternate Names for PUM1 Antibodies
anti-PUM1 antibody, anti-HSPUM antibody, anti-KIAA0099PUML1 antibody, anti-PUMH antibody, anti-PUMH1pumilio homolog 1 antibody, anti-pumilio (Drosophila) homolog 1 antibody, anti-pumilio homolog 1 (Drosophila) antibody, anti-pumilio-1 antibody
7 Results for "pum1" in Primary Antibodies
![Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0009.jpg "Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunoprecipitation-NB100-259-img0008.jpg)
![Western Blot: PUM1 Antibody [NBP2-20011] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Western-Blot-NBP2-20011-img0002.jpg "Western Blot: PUM1 Antibody [NBP2-20011] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP2-20011] - PUM1 antibody detects PUM1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUM1 stained by PUM1 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20011-img0005.jpg)
![Immunohistochemistry-Paraffin: PUM1 Antibody [NBP2-20011] - H441 xenograft, using Pumilio 1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20011-img0003.jpg)
![Western Blot: PUM1 Antibody [NBP2-20011] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Western-Blot-NBP2-20011-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP2-20011] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20011-img0004.jpg)
![Western Blot: PUM1 Antibody [NBP1-87856] - Analysis using Anti-PUM1 antibody NBP1-87856 (A) shows similar pattern to independent antibody NBP2-58337 (B).](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NBP1-87856-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUM1 Antibody [NBP1-87856] - Analysis using Anti-PUM1 antibody NBP1-87856 (A) shows similar pattern to independent antibody NBP2-58337 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP1-87856] - Staining of human cell line U-251 MG shows positivity in cytoplasm.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87856-img0005.jpg)
![Immunohistochemistry-Paraffin: PUM1 Antibody [NBP1-87856] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87856-img0006.jpg)
![Western Blot: PUM1 Antibody [NBP1-87856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NBP1-87856-img0007.jpg)
![Western Blot: PUM1 Antibody (8Y6H5) [NBP3-15279] - Western blot analysis of extracts of various cell lines, using PUM1 Rabbit mAb (NBP3-15279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PUM1-Antibody-8Y6H5-Western-Blot-NBP3-15279-img0002.jpg "Western Blot: PUM1 Antibody (8Y6H5) [NBP3-15279] - Western blot analysis of extracts of various cell lines, using PUM1 Rabbit mAb (NBP3-15279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: PUM1 Antibody (8Y6H5) [NBP3-15279] - Immunofluorescence analysis of U-2 OS cells using PUM1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15279_rabbit-pum1-mab-8y6h5-72202512432057.jpg)
![Western Blot: PUM1 Antibody [NBP2-58337] - Analysis using Anti-PUM1 antibody NBP2-58337 (A) shows similar pattern to independent antibody NBP1-87856 (B).](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NBP2-58337-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUM1 Antibody [NBP2-58337] - Analysis using Anti-PUM1 antibody NBP2-58337 (A) shows similar pattern to independent antibody NBP1-87856 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP2-58337] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58337-img0001.jpg)