PTGDR Antibody (1052528) [Unconjugated]

PTGDR was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human PTGDR Monoclonal Antibody (Catalog # MAB11249) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1052528
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PTGDR Antibody (1052528) [Unconjugated] Summary

• Immunogen: Synthetic peptide within amino acids 50-150 of the Prostaglandin D2 receptor protein sequences
  Accession # Q13258
• Specificity: Detects Prostaglandin D2 receptor immunogen in Direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTGDR Antibody (1052528) [Unconjugated]

• AS1
• ASRT1
• DP
• DP1
• MGC49004
• PGD receptor
• PGD2 receptor
• prostaglandin D2 receptor (DP)
• prostaglandin D2 receptor
• Prostanoid DP receptor
• PTGDR
• PTGDR1

Background

PTGDR is a G protein-coupled receptor that has been shown to function as a prostanoid DP receptor. PTGDR is expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes. The activity of this receptor is mainly mediated by G-S proteins that stimulate adenylate cyclase resulting in an elevation of intracellular cAMP and Ca2+. Knockout studies in mice suggest that the ligand of PTGDR, prostaglandin D2 (PGD2), functions as a mast cell-derived mediator to trigger asthmatic responses. Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13258(Human)