CRTH-2/GPR44 Antibody

Images

 
Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: CRTH-2/GPR44 Antibody [NBP1-76755] - Human Heart tissue with CRTH2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-76755] - Human heart tissue with GPR44 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, GPSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CRTH-2/GPR44 Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2.
Specificity
At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTGDR2
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 25917171).
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Publications
Read Publications using
NBP1-76755 in the following applications:

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 25917171).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CRTH-2/GPR44 Antibody

  • CD294 antigen
  • CD294
  • Chemoattractant receptor-homologous molecule expressed on Th2 cells
  • CRTH2
  • CRTH-2
  • CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
  • DL1R
  • DP2
  • G protein-coupled receptor 44
  • GPR44
  • PGD2R
  • PTGDR2
  • putative G-protein coupled receptor 44

Background

The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRTH-2/GPR44 Antibody (NBP1-76755)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTGDR2
Entrez
Uniprot