PSAP Antibody - BSA Free

Western Blot: PSAP Antibody [NBP1-33459] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-33459] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Western Blot: PSAP Antibody [NBP1-33459] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: PSAP Antibody [NBP1-33459] - Various extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (NBP1-33459) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: PSAP Antibody [NBP1-33459] - Wild-type (WT) and PSAP knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSAP Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.
• Localization: Lysosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (87%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PSAP Antibody - BSA Free

• FLJ00245
• GLBA
• proactivator polypeptide
• prosaposin
• PSAP
• SAP1
• SAP1MGC110993
• sphingolipid activator protein-1

Background

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PSAP