PSAP Antibody

Images

 
Western Blot: PSAP Antibody [NBP1-33459] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-33459] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: PSAP Antibody [NBP1-33459] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.97 mg/ml

Order Details

PSAP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.
Localization
Lysosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (87%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.97 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSAP Antibody

  • FLJ00245
  • GLBA
  • proactivator polypeptide
  • prosaposin
  • PSAP
  • SAP1
  • SAP1MGC110993
  • sphingolipid activator protein-1

Background

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSAP
Entrez