PSAP Antibody [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) followed ...read more
PSAP was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) at 1:100 dilution for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PSAP Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human PSAP
Gly17-Asn524
Accession # P07602
Specificity
Detects human PSAP in direct ELISAs and Western blots. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
  • Immunocytochemistry 1:100 dilution
Control
PSAP Knockout HeLa Cell Lysate
PSAP Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
AF8520 in the following applications:

Publications
Read Publication using
AF8520 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. See Certificate of Analysis for details.
Preservative
Sodium Azide
Purity
Immunogen affinity purified

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PSAP Antibody [Unconjugated]

  • FLJ00245
  • GLBA
  • proactivator polypeptide
  • prosaposin
  • PSAP
  • SAP1
  • SAP1MGC110993
  • sphingolipid activator protein-1

Background

Prosaposin behaves as a myelinotrophic and neurotrophic factor, whose effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.By similarity Saposins are specific low-molecular mass non-enzymic proteins and participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. The Prosaposin gets cleaved into 5 chains, Saposin A, B, B-Val, C and D. It also contains several disulfide bonds as well as glycosylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSAP Antibody (AF8520)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Immunoprecipitation.


Filter By Application
Immunoprecipitation
(1)
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Filter By Species
Human
(1)
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Review for PSAP Antibody (AF8520) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF8520:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PSAP AF8520
Enlarge
5
reviewed by:
Anonymous
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedA549 cells,H441
SpeciesHuman
LotCJCW0115011

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PSAP Antibody (AF8520) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for PSAP Antibody (AF8520)

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Visit Tool

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Blogs on PSAP

There are no specific blogs for PSAP, but you can read our latest blog posts.
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Contact Information

Learn the difference between western blot and simple western

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol PSAP
Uniprot