PRRX1 Antibody (CL14471) - Azide and BSA Free

Staining in U-138MG cell line with Anti-PRRX1 monoclonal antibody) .

Analysis in human cervix, uterine and liver tissues using NBP3-44310 antibody. Corresponding PRRX1 RNA-seq data are presented for the same tissues.

Staining of human endometrium shows strong nuclear positivity in glandular cells.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human prostate shows strong nuclear positivity in glandular cells.

Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL14471
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PRRX1 Antibody (CL14471) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PRRX1 Antibody (CL14471) - Azide and BSA Free (NBP3-26856) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P54821, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRRX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PRRX1 Antibody (CL14471) - Azide and BSA Free

• Homeobox protein PHOX1
• paired mesoderm homeo box 1
• paired mesoderm homeobox 1 isoform pmx-1b
• paired mesoderm homeobox protein 1
• paired related homeobox 1
• Paired-related homeobox protein 1
• PHOX1PRX-1
• PMX1PRX1

Background

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRRX1