Peroxiredoxin 6 Antibody (3A10-2A11) - Azide and BSA Free

Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.

Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.

ELISA: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - Detection limit for recombinant GST tagged PRDX6 is 3 ng/ml as a capture antibody.

• Reactivity: Hu, Bv
• Applications: WB, DB, ELISA
• Clone: 3A10-2A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Peroxiredoxin 6 Antibody (3A10-2A11) - Azide and BSA Free Summary

• Immunogen: PRDX6 (AAH35857.1, 1 a.a. ~ 224 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP
• Specificity: PRDX6 - peroxiredoxin 6
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Dot blot reported in scientific literature (PMID: 26344128).

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26344128).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Peroxiredoxin 6 Antibody (3A10-2A11) - Azide and BSA Free

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 6 Antibody (H00009588-M01)(12)

Publications using H00009588-M01

• Brigitte P, Arnaud C, Sebastien C et al. Relationships between the abundance of 29 proteins and several meat or carcass quality traits in two bovine muscles revealed by a combination of univariate and multivariate analyses. J Proteomics. 2022-12-16 [PMID: 36535620]
• Picard B, Gagaoua M, Al Jammas M, Bonnet M et al. Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices. J Proteomics. 2019-03-17 [PMID: 30894324]
• Romanello KS, Teixeira KKL, Silva JPMO et al. Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia. PLoS One 2018-12-06 [PMID: 30521599]
• Gagaoua M, Bonnet M, De Koning L et al. Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed. Meat Sci 2018-07-05 [PMID: 30015160]
• Picard B, Gagaoua M, Al-Jammas M et al. Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect. PeerJ 2018-06-07 [PMID: 29892502]
• Gagaoua M, Bonnet M, Ellies-Oury MP et al. Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses. Food Chem 2018-01-10 [PMID: 29412918]
• Gagaoua M, Terlouw EMC, Picard B. et al. The study of protein biomarkers to understand the biochemical processes underlying beef color development in young bulls. Meat Sci 2017-07-18 [PMID: 28750331]
• Gagaoua M, Terlouw EMC, Boudjellal A, Picard B. Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal. Journal of proteomics 2015-10-14 [PMID: 26344128] (WB, Cytometric Bead Assay Standard, Bovine)
• Picard B, Gagaoua M, Micol D et al. Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle. J Agric Food Chem 2014-08-24 [PMID: 25175407]
• Guillemin N, Bonnet M, Jurie C et al. Functional analysis of beef tenderness. J Proteomics 2011-08-09 [PMID: 21855665]
• Taga H, Chilliard Y, Meunier B et al. Cellular and molecular large-scale features of fetal adipose tissue: is bovine perirenal adipose tissue brown?. J Cell Physiol. 2012-04-01 [PMID: 21678425]
• Jia X, Veiseth-Kent E, Grove H et al. Peroxiredoxin-6--a potential protein marker for meat tenderness in bovine longissimus thoracis muscle. J Anim Sci. 2009-04-09 [PMID: 19359513]

Bioinformatics

• Gene Symbol: PRDX6
• Entrez:
  • 9588(Human)
• OMIM:
  • 602316(Human)
• Uniprot:
  • AAH35857(Human)