SUG1 Antibody (25D5)

Images

 
Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000
Genetic Strategies: Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] - Representative immunoblot for SUG1 antibody knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IP, KD
Clone
25D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

SUG1 Antibody (25D5) Summary

Immunogen
Recombinant protein encoding full-length SUG1 purified from E. coli.
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMC5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:1000
Application Notes
IP reactivity reported in (PMID: 19660582). SUG1 antibody validated for CHIP from a verified customer review.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-345 in the following applications:

Publications
Read Publications using
NB100-345 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for SUG1 Antibody (25D5)

  • MSUG1 protein
  • p45
  • p45/SUG26S protease regulatory subunit 8
  • proteasome (prosome, macropain) 26S subunit, ATPase, 5
  • proteasome 26S ATPase subunit 5
  • Proteasome 26S subunit ATPase 5
  • Proteasome subunit p45
  • S8
  • SUG-1
  • SUG126S proteasome AAA-ATPase subunit RPT6
  • Tat-binding protein homolog 10
  • TBP10
  • thyroid receptor interactor 1
  • TRIP1Thyroid hormone receptor-interacting protein 1

Background

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87797
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB
NBP1-80959
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-82761
Species: Hu
Applications: IHC, IHC-P
H00006192-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP1-82580
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
H00006201-M03
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP2-73636
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: CyTOF-ready, Flow, WB
NBP2-67301
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00006193-M02
Species: Hu, Mu, Pr, Rt
Applications: ELISA, ICC/IF, WB
NBP2-33717
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-38323
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-38780
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-88192
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-46191
Species: Dr, Hu, Mu
Applications: IP, WB
NBP2-94502
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-2682
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-CS, Flow, ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-81545
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for SUG1 Antibody (NB100-345)(4)

Reviews for SUG1 Antibody (NB100-345) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-345:
Filter by Applications
WB
(1)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot SUG1 NB100-345
Enlarge
5
reviewed by:
Verified Customer
WB Human 05/07/2013
View

Summary

ApplicationWestern Blot
SpeciesHuman
  3
reviewed by:
Verified Customer
ChIP Human 03/02/2010
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHuman Whole Cell lysate
SpeciesHuman
CommentsIt works well with both ChIP and WB. I am happy wit the Ab.

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: Undiluted, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Method: Real-time PCR, ChIP Type: Cross-linking (X-ChIP), Positive Control: IgG binding

Comments

CommentsIt works well with both ChIP and WB. I am happy wit the Ab.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SUG1 Antibody (NB100-345) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Verified Customer
05/07/2013
Application: WB
Species: Human

 
Verified Customer
03/02/2010
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol PSMC5
Entrez
Uniprot