SUG1 Antibody (25D5)

Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000

Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] - Representative immunoblot for SUG1 antibody knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC3695522/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, IP, ChIP, KD
• Clone: 25D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SUG1 Antibody (25D5) Summary

• Immunogen: Recombinant protein encoding full-length SUG1 purified from E. coli.
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMC5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:1000
• Application Notes: IP reactivity reported in (PMID: 19660582). SUG1 antibody validated for CHIP from a verified customer review.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SUG1 Antibody (25D5)

• MSUG1 protein
• p45
• p45/SUG26S protease regulatory subunit 8
• proteasome (prosome, macropain) 26S subunit, ATPase, 5
• proteasome 26S ATPase subunit 5
• Proteasome 26S subunit ATPase 5
• Proteasome subunit p45
• S8
• SUG-1
• SUG126S proteasome AAA-ATPase subunit RPT6
• Tat-binding protein homolog 10
• TBP10
• thyroid receptor interactor 1
• TRIP1Thyroid hormone receptor-interacting protein 1

Background

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUG1 Antibody (NB100-345)(4)

Publications using NB100-345

• Im E, Yoon JB, Lee HW, Chung KC. Human Telomerase Reverse Transcriptase (hTERT) Positively Regulates 26S Proteasome Activity J. Cell. Physiol. 2016-09-20 [PMID: 27648923] (WB, Human)
• Maganti N, Moody TD, Truax AD et al. Nonproteolytic Roles of 19S ATPases in Transcription of CIITApIV Genes. PLoS ONE 2014-03-14 [PMID: 24625964] (WB, IP, Chemotaxis, Human)
• Concannon C, Lahue RS. The 26S proteasome drives trinucleotide repeat expansions. Nucleic Acids Res 2013-04-24 [PMID: 23620289] (WB, Human)
• Koues OI, Dudley RK, Mehta NT, Greer SF. The 19S proteasome positively regulates histone methylation at cytokine inducible genes. Biochim Biophys Acta 2009-11-01 [PMID: 19660582] (Chemotaxis, WB, IP, Human)

Reviews for SUG1 Antibody (NB100-345) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Claire Concannon WB Human 05/07/2013

Summary

• Application: Western Blot
• Species: Human

reviewed by: System Admin ChIP Human 03/02/2010

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Human Whole Cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: PSMC5
• Entrez:
  • 5705(Human)
• Uniprot:
  • P62195(Human)