Prox1 Antibody

Images

 
Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1
Immunocytochemistry/ Immunofluorescence: PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PROX1 Antibody [NBP1-18605] - Frozen sections of a mouse embryo at day 13.5 with anti-CD31 [green] and anti-human Prox-1 [red]. It shows a large lymphatic vessel (lv), blood ...read more
Immunocytochemistry/ Immunofluorescence: PROX1 Antibody [NBP1-18605] - Frozen sections of a lymphangiom with anti-CD31 [green] and anti-human Prox-1 [red].

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Prox1 Antibody Summary

Immunogen
Recombinant human C-terminal part of Prox1 (homeo domain and prospero domain)
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROX1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:750-1:1000
  • Immunohistochemistry-Frozen
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 27122034).
Reviewed Applications
Read 1 Review rated 3
using
NBP1-18605 in the following applications:

Publications
Read Publications using
NBP1-18605 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27122034)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
5mM PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge prior to opening. Reconstitute with sterilized water to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Prox1 Antibody

  • Homeobox prospero-like protein PROX1
  • prospero homeobox 1
  • prospero homeobox protein 1
  • prospero-related homeobox 1
  • Prox1
  • PROX-1

Background

Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prox1 Antibody (NBP1-18605)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.


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ICC/IF
(1)
IHC-Fr
(1)
All Applications
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Human
(1)
Mouse
(1)
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Review for Prox1 Antibody (NBP1-18605) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-18605:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Prox1 NBP1-18605
Enlarge
reviewed by:
Anonymous
IF Human 11/06/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedhuman skin
SpeciesHuman
CommentsI had an enormous problem with this antibody before being able to see labelling, and all my tests showed no results, no signal at all, before this one. I decided then to use my last aliquots, as concentrated as possible (I diluted according to Novus suggestion, to 1mg/mL), and incubate for a long period, following "desperate" instrucions from a forum. As can be seen in the image, it worked, but I strongly recommend for future buyers to use and dilute the antibody in much higher concentrations than the ones Novus recommended. I spent all the vial trying many times, it was really time consuming and now, if purchasing again, I will have to reset the reactions. Still, it was good to see it working properly and I had great customer service concerning this problem with Prox-1.

Blocking

Blocking Details1h at RT with buffer containing PBS- normal goat serum 5%

Primary Anitbody

Dilution Ratio1:10 48h incubation

Secondary Antibody

Secondary Descriptiongoat anti rabbit
Secondary Manufacturer Cat#Jackson Immunoresearch - 111-166-045
Secondary Concentration1:1000

Details

Detection NotesLSM Meta 500 Zeiss Confocal Microscope
Fixation DetailsParaformaldehyde fixation (4%) for 24h; Triton 0,3% permeabilization for 30min; PBS washing
Wash DescriptionPBS 3 times

Comments

CommentsI had an enormous problem with this antibody before being able to see labelling, and all my tests showed no results, no signal at all, before this one. I decided then to use my last aliquots, as concentrated as possible (I diluted according to Novus suggestion, to 1mg/mL), and incubate for a long period, following "desperate" instrucions from a forum. As can be seen in the image, it worked, but I strongly recommend for future buyers to use and dilute the antibody in much higher concentrations than the ones Novus recommended. I spent all the vial trying many times, it was really time consuming and now, if purchasing again, I will have to reset the reactions. Still, it was good to see it working properly and I had great customer service concerning this problem with Prox-1.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Prox1 Antibody (NBP1-18605) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on Prox1.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Calculators

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Recent Reviews

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Anonymous
11/06/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol PROX1
Entrez
Uniprot