Prox1 Antibody - Azide and BSA Free

Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale?=?30 um. n?=?3 brains. E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale?=?30 um. n?=?3 brains. E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic ( red arrows) in the meninges but nuclear in neurons ( blue arrows). DAPI marks the nuclei in blue. Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00401-019-02091-z) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Prox1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Prox1 Antibody - Azide and BSA Free (NBP1-18605) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Prox1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Prox-1
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PROX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:750 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1-5 ug/ml
• Application Notes: Use in IHC-Frozen reported in scientific literature (PMID 27122034). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 31696318).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27122034).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.5X PBS, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for Prox1 Antibody - Azide and BSA Free

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prox1 Antibody (NBP1-18605)(5)

Publications using NBP1-18605

• Redondo PAG, Gubert F, Zaverucha-do-Valle C et al. Lymphatic vessels in human adipose tissue Cell Tissue Res. 2019-11-27 [PMID: 31776824] (ICC/IF, Human)
• Shibata-Germanos S, Goodman JR, Grieg A et al. Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges Acta Neuropathol. 2019-11-06 [PMID: 31696318] (IHC-P, Human)
• Rainey RN, Ng SY, Llamas J et al. Mutations in Cockayne Syndrome-Associated Genes (Csa and Csb) Predispose to Cisplatin-Induced Hearing Loss in Mice. J. Neurosci. 2016-04-27 [PMID: 27122034] (IHC-Fr, Mouse)
• Abdolazimi Y, Stojanova Z, Segil N. Selection of cell fate in the organ of Corti involves the integration of Hes/Hey signaling at the Atoh1 promoter. Development. 2016-03-01 [PMID: 26932672] (ICC/IF, Human)
• Du W, Ayyaswamy S, Rath S et Al. MEK/ERK inhibitor effectively impact generalized lymphatic anomaly (GLA) cells growth through EGFR/MEK/ERK signaling pathway assets.researchsquare.com 2020-01-01

Review for Prox1 Antibody (NBP1-18605) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 11/06/2014

Summary

• Application: Immunofluorescence
• Sample Tested: human skin
• Species: Human

Bioinformatics

• Gene Symbol: PROX1
• Entrez:
  • 5629(Human)
• Uniprot:
  • Q92786()