Coronin-1a Antibody

Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.

Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58867] - Analysis of cortical neuron-glial cell culture from E20 rat stained with Coronin-1a Antibody, dilution 1:1,000 (Red), and costained with mouse mAb to GFAP, dilution 1:1,000 (Green). Hoechst staining of nuclear DNA (Blue). The Coronin-1a Antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Coronin-1a Antibody Summary

• Immunogen: C-terminal peptide of human coronin 1a coupled to KLH. [UniProt# P31146]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CORO1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
• Application Notes: This Coronin-1a antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Coronin-1a Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Coronin-1a Antibody

• CLIPINA
• clipin-A
• CORO1
• coronin, actin binding protein, 1A
• coronin, actin-binding protein, 1A
• coronin-1
• coronin-1A
• Coronin-like protein A
• Coronin-like protein p57
• FLJ41407
• HCORO1
• MGC117380
• p57
• TACOCLABP
• Tryptophan aspartate-containing coat protein

Background

Highly conserved coronin family of proteins/actin regulators from WD-repeat superfamily were originally identified in Dictyostelium, wherein, coronin deletions were observed to affect actin-dependent processes, such as phagocytosis, cell motility, and cytokinesis. Coronin-1a (Coro1A) belongs to Type I coronins, almost exclusively expressed in hematopoietic lineages, and plays a key role during T lymphocyte activation, migration, survival, and calcium signaling. Moreover, Coronin-1a has been documented to be dispensable for actin-dependent functions in macrophages. Coro1a-deficient mice have reduced peripheral T cells due to both an impaired survival of naive cells and migrational defects. Both CD4+ and CD8+ T cell subsets are decreased in the periphery of Coro1a-/- mice while thymic cellularity and subpopulations are similar to those observed in wild-type mice. A nonsense mutation in Coro1a has been shown to protect against systemic lupus in a mouse model. Moreover, it has shown that Coro1a is mutated in a thymic egress-deficient mouse strain, but also in a patient with severe combined immunodeficiency (SCID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coronin-1a Antibody (NB110-58867)(2)

Publications using NB110-58867

• Tweedell R, Tao D, Dinglasan RR. The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages. Proteomics. 2014 Sep 30 [PMID: 25266554] (WB, ICC/IF, Rat)
  
  Details:
  Coronin-1a antibody used for WB at 1:5000 dilution on lysates of RKC1/rat Kupffer cell line exposed or not to LPS (Figure 2A). Antibody also used for ICC-IF at 1:500 dilution on primary rat Kupffer cells and RKC1 cells with or without LPS stimulation.
• Stray-Pedersen A, Jouanguy E, Crequer A et al. Compound Heterozygous CORO1A Mutations in Siblings with a Mucocutaneous-Immunodeficiency Syndrome of Epidermodysplasia Verruciformis-HPV, Molluscum Contagiosum and Granulomatous Tuberculoid Leprosy. J. Clin. Immunol. 2014 Jul 30 [PMID: 25073507] (WB, Human)
  
  Details:
  Coronin-1a antibody used in WB for detecting CORO1A clinical phenotype/ deficiency of coronin-1A protein in study involving siblings with a mucocutaneous-immunodeficiency syndrome of spidermodysplasia verruciformis-HPV, molluscum contagiosum and granulomatous tuberculoid leprosy. Lysates of lymphoblastoid/lymphoid cell lines from P2 (affected sibling) and P3/P4 (parental carriers) were prepared in 1 % NP-40 lysis buffer/HALT protease inhibitor, blot blocked with 3% skim milk followed by primary incubation and detection with Odyssey/Licor method (Fig 6 - Coronin-1a band size 57kDa; Loading control used Myosin IIA).

Bioinformatics

• Gene Symbol: CORO1A
• Entrez:
  • 11151(Human)
• Uniprot:
  • P31146(Human)