Protein Phosphatase 1C gamma Antibody

Images

 
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Protein Phosphatase 1C gamma Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PP1C gamma. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1CC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Application Notes
IP usage reported in scientific literature (PMID: 24722282).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32858 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%), Expected cross reactivity based on sequence homology: Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein Phosphatase 1C gamma Antibody

  • EC 3.1.3.16
  • PP-1G
  • PP1gamma
  • PPP1G
  • protein phosphatase 1, catalytic subunit, gamma isoform
  • protein phosphatase 1, catalytic subunit, gamma isozyme
  • Protein phosphatase 1C catalytic subunit
  • serine/threonine phosphatase 1 gamma
  • serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Background

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The protein is involved in regulation of ionic conductances and long term synaptic plasticity. It may play an important role in dephosphorylating substrates such as the postsynaptic density associated Ca (2+)/calmodulin dependent protein kinase II. PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC (PP1C gamma), which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with cyanobacterial toxin microcystin; disulfide-linked. Interacts with PPP1R7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protein Phosphatase 1C gamma Antibody (NBP1-32858)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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(1)
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(1)
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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPP1CC