PP1 alpha/PPP1A Antibody (OTI6E5)

Western Blot: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1CA.

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Human liver tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody (OTI6E5) [NBP2-45384] - Staining of paraffin-embedded Human pancreas tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clone: OTI6E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PP1 alpha/PPP1A Antibody (OTI6E5) Summary

• Immunogen: Full length human recombinant protein of human PPP1CA(NP_001008709) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1CA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 38.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PP1 alpha/PPP1A Antibody (OTI6E5)

• EC 3.1.3.16
• PP1 alpha
• PP1alpha
• PP-1AMGC15877
• PPP1A
• PPP1AMGC1674
• PPP1CA
• protein phosphatase 1, catalytic subunit, alpha isoform
• protein phosphatase 1, catalytic subunit, alpha isozyme
• serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
• serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1CA