ARK5/NUAK1 Antibody (715901)

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Western blot shows lysates of Saos‑2 human osteosarcoma cell line, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ARK5/NUAK1 Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
715901
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

ARK5/NUAK1 Antibody (715901) Summary

Immunogen
E. coli-derived recombinant human ARK5/NUAK1
Lys311-Met462
Accession # O60285
Specificity
Detects human ARK5/NUAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SNARK is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NUAK1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARK5/NUAK1 Antibody (715901)

  • AMPK-related protein kinase 5
  • ARK5
  • ARK5AMP-activated protein kinase family member 5
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA0537NUAK family SNF1-like kinase 1
  • NUAK family, SNF1-like kinase, 1
  • NUAK1

Background

AMPK-related kinase 5 (ARK5), also known as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short isoform of the human protein is prematurely tuncated within this domain. Within aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NUAK1
Uniprot