Protein Disulfide Isomerase/P4HB Antibody [Unconjugated]

Images

 
Western blot shows lysates of PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Protein Disulfide Isomerase/P4HB ...read more
Simple Western lane view shows lysates of PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Protein Disulfide ...read more
Protein Disulfide Isomerase/P4HB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Protein Disulfide Isomerase/P4HB Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Protein Disulfide Isomerase/P4HB Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human P4HB
Asp18-Lys505
Accession # P07237
Specificity
Detects human P4HB in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
P4HB
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunocytochemistry 1-15 ug/mL
Positive Control
Protein Disulfide Isomerase/P4HB Lysate (NBP2-64836)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody [Unconjugated]

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4 hydroxylase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491 amino acids (aa) in length. It contains two TRX domains (aa 25 134 and 368 475) plus an ER retention sequence (aa 505 508). There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18 505, human P4HB shares 94% aa identity with mouse P4HB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol P4HB
Entrez
Uniprot