Prolactin Antibody [Unconjugated]


Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) followed by more
Prolactin was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) at 1 µg/mL overnight at 4 °C. Tissue more
Recombinant Human Prolactin (Catalog # 682-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Prolactin more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)

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Prolactin Antibody [Unconjugated] Summary

E. coli-derived recombinant human Prolactin
Accession # Q5THQ0
Detects human Prolactin in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse Prolactin and recombinant human Prolactin R.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 1-15 ug/mL
  • Neutralization 0.02-0.05 ug/mL
  • Western Blot 0.25 ug/mL
Application Notes
ELISA Capture: Human Prolactin Antibody (Catalog # AF682)
ELISA Detection: Human Prolactin Biotinylated Antibody (Catalog # BAF682)
Standard: Recombinant Human Prolactin (Catalog # 682-PL)
Read Publications using
AF682 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prolactin Antibody [Unconjugated]

  • PRL
  • Prolactin


Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cell, T cells, NK cells, and breast cancer cells. Originally characterized as a lactogenic hormone, studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. In the immune system, prolactin has been shown to be secreted by human PBMC and to act as a proliferative growth factor. Additionally, prolactin treatment of human PBMC has been shown to enhance IFN-gamma production. Prolactin has several molecular forms. The predominant form is a monomer, the non-glycosylated form is 23 kDa and the glycosylated form is 25 kDa. Glycosylated prolactin is removed from the circulation faster and has been reported to have lower biological potency. Prolactin cDNA encodes a 227 amino acid residue protein with a putative 28 aa residue signal peptide. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. B cells, T cells, macrophages, NK cells, monocytes, CD34+ progenitor cells, neutrophils, mammary gland, liver, kidney, adrenals, ovaries, testis, prostrate, seminal vesicles, and hypothalamus have all been shown to express the prolactin receptor. Three forms of the receptor, generated by differential splicing, have been identified. These isoforms differ in the length of their cytoplasmic domains. It is believed that the short cytoplasmic form is non-functional. Prolactin signal transduction involves the JAK/STAT families and Src kinase family.

  1. Cooke, N.E. et al. (1981) J. Biol. Chem. 256:4007.
  2. Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639.
  3. Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89.
  4. Price, A.E. et al. (1995) Endoc. 136:4827.
  5. Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
  6. Bellone, G. et al. (1995) J. Cell Physiol. 163:221.
  7. Cole, E. et al. (1991) Endoc. 129:2639.
  8. Lewis, U. et al. (1985) Endoc. 116:359.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin Antibody (AF682)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, IHC, Neutralization, Western Blot.

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Western Blot
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Showing Publications 1 - 6 of 6.
Publications using AF682 Applications Species
S Löb, B Ochmann, Z Ma, T Vilsmaier, C Kuhn, E Schmoeckel, SL Herbert, T Kolben, A Wöckel, S Mahner, U Jeschke The role of Interleukin-18 in recurrent early pregnancy loss Journal of reproductive immunology, 2021-10-05;148(0):103432. 2021-10-05 [PMID: 34627076] (IHC, Human) IHC Human
S Löb, N Amann, C Kuhn, E Schmoeckel, A Wöckel, A Zati Zehni, T Kaltofen, S Keckstein, JN Mumm, S Meister, T Kolben, S Mahner, U Jeschke, T Vilsmaier Interleukin-1 beta is significantly upregulated in the decidua of spontaneous and recurrent miscarriage placentas Journal of reproductive immunology, 2021-01-30;144(0):103283. 2021-01-30 [PMID: 33545613] (IHC, Human) IHC Human
R Sudharsan, L Murgiano, HY Tang, TW Olsen, VRM Chavali, GD Aguirre, WA Beltran Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration Scientific Reports, 2021-01-11;11(1):460. 2021-01-11 [PMID: 33432105] (Western Blot, Human) Western Blot Human
Kanda N, Watanabe S Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes. Endocrinology, 2007-01-25;148(5):2317-25. 2007-01-25 [PMID: 17255201] (ELISA Development, Human) ELISA Development Human
Lange T, Dimitrov S, Fehm HL, Westermann J, Born J Shift of monocyte function toward cellular immunity during sleep. Arch. Intern. Med., 2006-09-18;166(16):1695-700. 2006-09-18 [PMID: 16983046] (Neutralization, Human) Neutralization Human
Chakravarti P, Henry MK, Quelle FW Prolactin and heregulin override DNA damage-induced growth arrest and promote phosphatidylinositol-3 kinase-dependent proliferation in breast cancer cells. Int. J. Oncol., 2005-02-01;26(2):509-14. 2005-02-01 [PMID: 15645137] (Neutralization, Human) Neutralization Human

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Gene Symbol PRL