Prolactin Antibody (PRL/4908R)

Immunohistochemistry-Paraffin: Prolactin Antibody (PRL/4908R) [NBP3-07241] - Formalin-fixed, paraffin-embedded human pituitary carcinoma stained with Prolactin Recombinant Rabbit Monoclonal Antibody (PRL/4908R).

Immunohistochemistry-Paraffin: Prolactin Antibody (PRL/4908R) [NBP3-07241] - Formalin-fixed, paraffin-embedded human pituitary stained with Prolactin Recombinant Rabbit Monoclonal Antibody (PRL/4908R).

Immunohistochemistry-Paraffin: Prolactin Antibody (PRL/4908R) [NBP3-07241] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Prolactin antibody (PRL/4908R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: PRL/4908R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Prolactin Antibody (PRL/4908R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08249)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Prolactin (PRL) protein (around aa 63-201) (exact sequence is proprietary) (Uniprot: IDP01236)
• Localization: Secreted.
• Marker: Pituitary Tumor Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Prolactin Antibody (PRL/4908R)

• PRL
• Prolactin

Background

Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRL