PPAR gamma/NR1C3 Antibody - BSA Free

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223] - The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (kDa) is shown on the left.

ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPAR gamma/NR1C3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PPAR gamma/NR1C3 Antibody - BSA Free (NBP2-59223) is a polyclonal antibody validated for use in WB, ELISA and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARG
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1 ug
  • ELISA 1:1000
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Peptide affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody - BSA Free

• CIMT1
• NR1C3
• NR1C3GLM1
• Nuclear receptor subfamily 1 group C member 3
• peroxisome proliferator-activated receptor gamma 1
• peroxisome proliferator-activated receptor gamma
• PPAR gamma
• PPARG
• PPARG1peroxisome proliferative activated receptor, gamma
• PPARG2PPARgamma
• PPAR-gamma

Background

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPARG