PPAR gamma/NR1C3 [p Ser273] Antibody - BSA Free

ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159] - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate.

• Reactivity: Hu
• Applications: DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPAR gamma/NR1C3 [p Ser273] Antibody - BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma/NR1C3 at the Ser273 residue
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PPAR gamma/NR1C3 [p Ser273] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein. (Uniprot: P37231)
• Modification: p Ser273
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000
• Application Notes: This product has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 [p Ser273] Antibody - BSA Free

• CIMT1
• NR1C3
• NR1C3GLM1
• Nuclear receptor subfamily 1 group C member 3
• peroxisome proliferator-activated receptor gamma 1
• peroxisome proliferator-activated receptor gamma
• PPAR gamma
• PPARG
• PPARG1peroxisome proliferative activated receptor, gamma
• PPARG2PPARgamma
• PPAR-gamma

Background

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPARG
• Uniprot:
  • P37231(Human)