PPAR gamma/NR1C3 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, HaSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PPAR gamma/NR1C3 Antibody Summary

Immunogen
Synthetic peptide corresponding to the C terminus of human PPAR gamma.
Localization
Nuclear
Specificity
Will recognize both PPARgamma1 and PPARgamma2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
IHC-P is described in PMID: 25187315.
Reviewed Applications
Read 1 Review rated 5
using
NB120-19481 in the following applications:

Publications
Read Publications using
NB120-19481 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS with 0.2% gelatin
Preservative
0.1% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Peroxisome proliferator-activated receptors (PPAR) are members of the steroid nuclear receptor superfamily (1). The mammalian PPARs include three subtypes PPARalpha, PPARbeta, and PPARgamma. PPARs have been found to regulate fatty acid oxidation, fat cell development, lipoprotein metabolism, and glucose homeostasis (2). Recent studies have found that PPARs can affect the pathogenesis and development of tumors (3). PPARgamma and PPARbeta have been specifically implicated in tumorigenesis (3). PPARalpha is the focus of research studying its function in fatty acid beta-oxidation, lipid metabolism, and vascular inflammation (4). PPARbeta also plays a key role in the activation of keratinocytes during the inflammatory reaction associated with a skin injury (5). Of the three subtypes of PPARs, PPARgamma is the most widely studied. PPARgamma has been implicated in various human chronic diseases such as diabetes mellitus, atherosclerosis, rheumatoid arthritis, inflammatory bowel disease, and Alzheimer's disease (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR gamma/NR1C3 Antibody (NB120-19481)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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Human
(1)
Rat
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Review for PPAR gamma/NR1C3 Antibody (NB120-19481) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB120-19481:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 12/20/2011
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Summary

ApplicationWestern Blot
Sample TestedMouse BALB/c macrophage cell line
SpeciesMouse
CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:400, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Rabbit IgG HRP, Jackson

Details

Detection NotesDetection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPAR gamma/NR1C3 Antibody (NB120-19481). (Showing 1 - 2 of 2 FAQs).

  1. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
    • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
  2. Do you have a trial sample for this antibody. I want to use it for pig samples but I'm afraid it really works for pig samples.
    • Unfortunately we do not provide free samples however I can guarantee that this antibody has been tested on pig and if it does not work we will provide a full refund or replacement.

Secondary Antibodies

 

Isotype Controls

Additional PPAR gamma/NR1C3 Products

Bioinformatics Tool for PPAR gamma/NR1C3 Antibody (NB120-19481)

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Research Areas for PPAR gamma/NR1C3 Antibody (NB120-19481)

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Blogs on PPAR gamma/NR1C3.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

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Recent Reviews

5
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1
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Anonymous
12/20/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARG
Entrez