POT1 Antibody (688923)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human POT1 Monoclonal Antibody (Catalog # MAB5299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 688923
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

POT1 Antibody (688923) Summary

• Immunogen: E. coli-derived recombinant human POT1
  Met159-Pro316
  Accession # Q9NUX5
• Specificity: Detects human POT1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: POT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for POT1 Antibody (688923)

• DKFZp586D211
• HPOT1
• POT1 protection of telomeres 1 homolog
• POT1
• POT1-like telomere end-binding protein
• protection of telomeres 1 homolog (S. pombe)
• protection of telomeres protein 1

Background

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins.  It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes.  This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs.  Human POT1 is 634 amino acids (aa) in length.  It contains two N-terminal DNA binding OB regions (aa 1-300) and a C-terminal TPP-1 dimerization domain (aa 301-634).  There are multiple isoform variants.  There is a potential alternate start site at Met132, plus a three aa substitution for aa 43-634, a 24 aa substitution for aa 317-634, a three aa substitution for aa 457-634, and a 16 aa substitution for aa 503-634.  Over aa 159-316, human POT1 shares 71% aa identity with mouse POT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POT1 Antibody (MAB5299)(1)

Publication using MAB5299

• A Deregowska, M Pepek, K Pruszczyk, MM Machnicki, M Wnuk, T Stoklosa Differential Regulation of Telomeric Complex by BCR-ABL1 Kinase in Human Cellular Models of Chronic Myeloid Leukemia-From Single Cell Analysis to Next-Generation Sequencing Genes (Basel), 2020-09-29;11(10):. 2020-09-29 [PMID: 33003326] (Western Blot, Human)

Bioinformatics

• Gene Symbol: POT1
• Uniprot:
  • Q9NUX5()