POT1 Antibody (688923) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 688923
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

POT1 Antibody (688923) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human POT1
  Met159-Pro316
  Accession # Q9NUX5
• Specificity: Detects human POT1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for POT1 Antibody (688923) [Alexa Fluor® 647]

• DKFZp586D211
• hPot1
• POT1 protection of telomeres 1 homolog
• POT1
• POT1-like telomere end-binding protein
• protection of telomeres 1 homolog (S. pombe)
• protection of telomeres protein 1

Background

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins.  It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes.  This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs.  Human POT1 is 634 amino acids (aa) in length.  It contains two N-terminal DNA binding OB regions (aa 1-300) and a C-terminal TPP-1 dimerization domain (aa 301-634).  There are multiple isoform variants.  There is a potential alternate start site at Met132, plus a three aa substitution for aa 43-634, a 24 aa substitution for aa 317-634, a three aa substitution for aa 457-634, and a 16 aa substitution for aa 503-634.  Over aa 159-316, human POT1 shares 71% aa identity with mouse POT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9NUX5()