Podoplanin Antibody - Azide and BSA Free

Western Blot: Podoplanin Antibody [NB600-1014] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Podoplanin Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Podoplanin Antibody - Azide and BSA Free (NB600-1014) is a polyclonal antibody validated for use in IHC, WB, Flow and IP. Anti-Podoplanin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Podoplanin
• Localization: Type I membrane protein.
• Marker: Lymphatic Endothelium Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDPN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-5 ug/ml
• Application Notes: WB: Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry reactivity reported in (PMID:21406228).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for Podoplanin Antibody - Azide and BSA Free

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (NB600-1014)(1)

Publication using NB600-1014

• Steele M, Schieler A, Kelley P et al. b1 integrin regulates MMP-10 dependant tubulogenesis in human lymphatic endothelial cells Matrix Biol 2011-04-01 [PMID: 21406228] (FLOW, Human)

Bioinformatics

• Gene Symbol: PDPN
• Entrez:
  • 10630(Human)