Anti-Podoplanin Antibodies: Novus Biologicals

AF3244

Podoplanin Antibody [Unconjugated]

(1 Review)

100 μg

AF3244

HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Simple Western shows lysates of mouse lung, loaded at 0.5 mg/ml. A specific band was detected for Podoplanin at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Mfn1/2i delta AEC2 mice develop spontaneous lung fibrosis. a Schema demonstrating the generation of mice with AEC2 cell-specific tamoxifen-inducible deletion of Mfn1/2. SftpcCreERT2+/+ or SftpcCreERT2+/− mice were used as controls. b Genotyping of CD45(-)EpCAM(+) cells isolated from Mfn1/2i delta AEC2 mice (n = 3 mice; lane 1 to lane 3 serves as the positive control). c Representative immunoblots of AEC2 cell lysates obtained 6 weeks after tamoxifen-induced deletion, showing decreased protein levels of both MFN1 and MFN2 in the Mfn1/2−/− AEC2 cells (n = 3 mice per group). d Representative TEM images (upper row, ×12,000; lower row, ×50,000) show mitochondrial ultrastructural changes in SftpcCreERT2+/− and Mfn1/2−/− AEC2 cells (n = 3 mice per group) with disrupted cristae marked with white arrowheads (scale bar, upper row 2 μm, lower row 500 nm. e Kaplan–Meier survival curves of Mfn1/2i delta AEC2 (n = 22) and SftpcCreERT2+/+ (n = 23) mice (p < 0.01 by log-rank test). f Representative Masson’s trichrome-stained lung sections (upper panel, ×100 magnification; lower panel, ×200 magnification) 17 weeks post tamoxifen-induced deletion (SftpcCreERT2+/+ mice n = 6; Mfn1/2i delta AEC2 mice n = 11; scale bar, upper panel 4 mm, lower panel 200 μm). g Representative IHC staining of vimentin, alpha-smooth muscle actin ( alpha -SMA), and collagen III (Col-III) (×200 magnification; n = 3 mice per group; scale bar 200 μm). h Representative immunofluorescent staining of 5x5 tiled confocal images (using ×40 objective) of frozen murine lung sections stained for podoplanin (green), surfactant protein-C (SP-C) (yellow), ER-TR7 (magenta), and Hoechst 33342 stain (blue) (n = 3 mice per group; scale bar 50 μm). i Representative immunofluorescence staining confocal images of podoplanin (green), SP-C (yellow), ER-TR7 (magenta) and Hoechst 33342 nuclear stain (blue) using lung sections of SftpcCreERT2+/−, Mfn1i delta AEC2 and Mfn2i delta AEC2 mice (n = 2 mice per group; scale bar 20 μm). Source data (c, e) are provided as a Source Da

Effect of VEGFCC156S on angiotensin II-induced cardiac dysfunction, cardiac lymphatics, and skin lymphatics.(A) Pharmacokinetic analysis of VEGFCC156S in mice (n = 2 per dose group). (B) Plasma VEGFC concentration measured by ELISA at euthanasia (n = 10–17/group). (C–S) Mice were infused with saline + BSA, angiotensin II + BSA, or angiotensin II + VEGFCC156S as described in Figure 2A. (C) Representative M-mode echocardiography images for each group. (D–G) Echocardiography parameters cardiac output (D), stroke volume (E), Left Ventricular Posterior Wall thickness at diastole (LVPWd) (F) and heart rate (G) are shown (n = 15–24/group). (H–J) Hearts were arrested in diastole, fixed, sectioned, and stained with wheat germ agglutinin (WGA). (H) Representative images of WGA stain for each group. Scale bar, 100 μm. (I) Cardiomyocyte size was assessed (n = 7–9/group). (J) Analysis of cell size variance coefficient from WGA-stained mouse heart sections (n = 21–27/group; a linear mixed model was used for statistics). (K–N) Protein lysates were prepared from mouse hearts and immunoblotted for podoplanin, p-Akt, Akt, p-Erk1/2, Erk1/2, and GAPDH. (K) Representative immunoblots. (L–N) Densitometric quantification of podoplanin (L), p-Akt to Akt ratio (M) and p-Erk to Erk ratio (N) (n = 7–9/group). (O) Representative images of the whole mount stain of lyve1-positive lymphatic vessels in ear skin for each group. Scale bar, 100 μm. (P) Quantification of lyve1-positive lymphatic vessel diameter in ear skin (n = 737–880 vessel/group; a linear mixed model was used for statistics). (Q) Ear skin samples were fixed, sectioned, and stained for lyve1(green) and DAPI (blue). Representative images of lyve1-positive lymphatic vessels in ear skin cross-section for each group. Scale bar, 200 μm. (R) Quantification of lyve1-positive lymphatic vessel density (normalized to total area) in ear skin (n = 6–8 animals/group, n = 6–8 sections/animal; a linear mixed model was used for statistics). Ctrl, c

Carboplatin induces lymphangiogenesis in healthy tissues (A) Schematic of rat mesentery culture model. (B) Vehicle-treated lymphatic vessels from mesentery cultures stained with LYVE-1 (grey). (i) High magnification image of boxed area in (B). (C) Carboplatin-treated lymphatic vessels from mesentery cultures stained with LYVE-1 (grey). (ii) High magnification image of boxed area in (C) Scale bar=100µm. (D) Number of sprouts per lymphatic vessel area (n=3/group). (E) Lymphatic vessel density (podoplanin+ vessels per mm2 stroma) in whole mammary fat pads of healthy mice treated with systemic carboplatin (8 mg/kg/dose) or vehicle by IV(n=3-4/group). (F) Lymphatic vessel density measured in mammary fat pads of healthy mice 2 months after treatment with 3 doses of carboplatin or vehicle, (n=3/group). (G) Lymph nodes from healthy, tumor-naïve mice treated with vehicle and stained H&E. (H) LEC number in vehicle-treated and carboplatin-treated lymph nodes in vivo (n=6/group). (I) Representative images of lungs from mice treated with 3 doses of vehicle (left) and carboplatin (right). Podoplanin+ lymphatic vessels noted by arrowheads. (J) Lymphatic vessel density in stromal tissue of lungs of mice pre-treated with carboplatin (n=3/group). *p < 0.05, **p < 0.01, ****p < 0.001. Each data point represents one mouse. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35372032), licensed under a CC-BY license. Not internally tested by R&D Systems.

Podoplanin immunoreactivity in FFPE sections of mouse small intestine and lung. AF3244 was used at 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 20min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-goat HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.

Podoplanin IF staining of mouse lung tissue (Cy5, magenta). Image from a verified customer review.

Characterization of dermal lymphatic vessels in WT and Vegfr2Y949F/Y949F embryos and adult mice.(A) Dermal lymphatic vessels in E14.5 WT and Vegfr2Y949F/Y949F (Y949F) embryos shown by immunostaining of Neuropilin2 (Nrp2) in the back skin. Scale bar, 500 μm. (B) Quantification of dermal lymphatic sprouting tips/mm2 in the E14.5 embryos. WT, n = 13; Y949F, n = 10. NS, not significant, t test. (C) Lymphatic vessel density measured by NRP2 staining and normalized to tissue area/field of view in the E14.5 embryos. WT, n = 7; Y949F, n = 6. NS, not significant, t test. (D) Representative images of LYVE1 immunofluorescence showing ear dermal lymphatic vasculature in 8–10-wk-old mice. Scale bar, 1 mm. (E) Quantification of LYVE1+ lymphatic vessel width in 8–10-wk-old mouse ear dermis. n = 4 mice/genotype. NS, not significant, t test. (F) Quantification of LYVE1+ lymphatic vessel density in the 8–10-wk-old mouse ear dermis. WT, n = 4; Y949F, n = 3. P = 0.0087, t test. (G) Expressions of VEGFR2, VEGFR3, and Podoplanin in isolated LYVE1+ cells from lungs of WT and Vegfr2Y949F/Y949F mice at postnatal day 10 and in HUVECs, detected by immunoblotting. (H) Quantification of expression levels normalized to GAPDH in (G).Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38148112), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3244U-100UG
Alexa Fluor 405	AF3244V-100UG
Alexa Fluor 488	AF3244G-100UG
Alexa Fluor 532	AF3244X-100UG
Alexa Fluor 594	AF3244T-100UG
Alexa Fluor 647	AF3244R-100UG
Alexa Fluor 700	AF3244N-100UG
Alexa Fluor 750	AF3244S-100UG
Alexa Fluor Plus 405	AF3244AFP405-100UG
Alexa Fluor Plus 488	AF3244AFP488-100UG
Alexa Fluor Plus 555	AF3244AFP555-100UG
Alexa Fluor Plus 594	AF3244AFP594-100UG
Alexa Fluor Plus 647	AF3244AFP647-100UG
Alexa Fluor Plus 680	AF3244AFP680-100UG
Biotin	BAF3244

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3244-SP

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NB600-1015

Podoplanin Antibody (8.1.1) - BSA Free

(1 Review)

(24 Publications)

0.1 ml

NB600-1015

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin and these are cells of a malignant serosal tumor. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. <i>Part Fibre Toxicol</i> (2014)

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.

<b>Biological Strategies Validation. </b>Podoplanin-Antibody-8-1-1-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1015-img0013.jpg

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.

Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).

<b>Biological Strategies Validation. </b>Podolanin expressing on Mɸs mediates interactions with platelets.Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody ( alpha -podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Mɸs and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 μm.

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-11-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue). " class="big_lightbox">

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the <a class=

human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue). " class="big_thumb" />

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Golden Syrian Hamster Monoclonal

Host: Golden Syrian Hamster Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1015AF350
Alexa Fluor 405	NB600-1015AF405
Alexa Fluor 488	NB600-1015AF488
Alexa Fluor 532	NB600-1015AF532
Alexa Fluor 594	NB600-1015AF594
Alexa Fluor 647	NB600-1015AF647
Alexa Fluor 700	NB600-1015AF700
Alexa Fluor 750	NB600-1015AF750
Alexa Fluor Plus 405	NB600-1015AFP405
Alexa Fluor Plus 488	NB600-1015AFP488
Alexa Fluor Plus 555	NB600-1015AFP555
Alexa Fluor Plus 594	NB600-1015AFP594
Alexa Fluor Plus 647	NB600-1015AFP647
Alexa Fluor Plus 680	NB600-1015AFP680
Allophycocyanin	NB600-1015APC
Biotin	NB600-1015B
CoraFluor 1	NB600-1015CL1
DyLight 350	NB600-1015UV
DyLight 405	NB600-1015V
DyLight 488	NB600-1015G
DyLight 550	NB600-1015R
DyLight 594	NB600-1015DL594
DyLight 650	NB600-1015C
DyLight 680	NB600-1015FR
DyLight 755	NB600-1015IR
FITC	NB600-1015F
HRP	NB600-1015H
Janelia Fluor 525	NB600-1015JF525
Janelia Fluor 549	NB600-1015JF549
Janelia Fluor 646	NB600-1015JF646
Janelia Fluor 669	NB600-1015JF669
PE	NB600-1015PE
PerCP	NB600-1015PCP
mFluor Violet 450 SE	NB600-1015MFV450
mFluor Violet 500 SE	NB600-1015MFV500
mFluor Violet 610 SE	NB600-1015MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1015SS
Azide and BSA Free	NBP2-80916

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NB110-96423

Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

(8 Publications)

0.1 mg

NB110-96423

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 um. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti- Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both proteins in lymphatic endothelial cells. Note: The anti-mouse Lyve-1 polyclonal antibody shows a strong cross reaction with rat Lyve-1 protein.

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Sarcomatoid-type malignant mesothelioma observed in the MWCNT B high-dose group. A: H & E, low magnification of tumor tissue invading the diaphragm (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing several positive cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the MWCNT D low-dose group. A: H & E, low magnification of tumor tissue found on the surface of the omentum (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of the majority of cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-96423AF350
Alexa Fluor 405	NB110-96423AF405
Alexa Fluor 488	NB110-96423AF488
Alexa Fluor 532	NB110-96423AF532
Alexa Fluor 594	NB110-96423AF594
Alexa Fluor 647	NB110-96423AF647
Alexa Fluor 700	NB110-96423AF700
Alexa Fluor 750	NB110-96423AF750
Alexa Fluor Plus 405	NB110-96423AFP405
Alexa Fluor Plus 488	NB110-96423AFP488
Alexa Fluor Plus 555	NB110-96423AFP555
Alexa Fluor Plus 594	NB110-96423AFP594
Alexa Fluor Plus 647	NB110-96423AFP647
Alexa Fluor Plus 680	NB110-96423AFP680
Allophycocyanin	NB110-96423APC
Biotin	NB110-96423B
CoraFluor 1	NB110-96423CL1
DyLight 350	NB110-96423UV
DyLight 405	NB110-96423V
DyLight 488	NB110-96423G
DyLight 550	NB110-96423R
DyLight 594	NB110-96423DL594
DyLight 650	NB110-96423C
DyLight 680	NB110-96423FR
DyLight 755	NB110-96423IR
FITC	NB110-96423F
HRP	NB110-96423H
Janelia Fluor 525	NB110-96423JF525
Janelia Fluor 549	NB110-96423JF549
Janelia Fluor 646	NB110-96423JF646
Janelia Fluor 669	NB110-96423JF669
PE	NB110-96423PE
PerCP	NB110-96423PCP
mFluor Violet 450 SE	NB110-96423MFV450
mFluor Violet 500 SE	NB110-96423MFV500
mFluor Violet 610 SE	NB110-96423MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB110-96423SS
Azide and BSA Free	NBP2-80915

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AF3670

Podoplanin Antibody [Unconjugated]

(20 Publications)

100 μg

AF3670

HAF016). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of MG‑63 human osteosarcoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of MG‑63 human osteosarcoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Podoplanin was detected in immersion fixed paraffin-embedded sections of human uterus using 2 µg/mL Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3670U-100UG
Alexa Fluor 405	AF3670V-100UG
Alexa Fluor 488	AF3670G-100UG
Alexa Fluor 532	AF3670X-100UG
Alexa Fluor 594	AF3670T-100UG
Alexa Fluor 647	AF3670R-100UG
Alexa Fluor 700	AF3670N-100UG
Alexa Fluor 750	AF3670S-100UG
Alexa Fluor Plus 405	AF3670AFP405-100UG
Alexa Fluor Plus 488	AF3670AFP488-100UG
Alexa Fluor Plus 555	AF3670AFP555-100UG
Alexa Fluor Plus 594	AF3670AFP594-100UG
Alexa Fluor Plus 647	AF3670AFP647-100UG
Alexa Fluor Plus 680	AF3670AFP680-100UG
Allophycocyanin	FAB3670A
Biotin	BAF3670

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3670A
Carrier Free	AF3670-SP

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NBP2-03952

Podoplanin Antibody (NZ-1.2) - BSA Free

(3 Publications)

0.1 mg

NBP2-03952

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A surface stain was performed on Ntera2 cells with Podoplanin Antibody (NZ-1.2) NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-6742A

Alt Catalog# IMG-6742A

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-03952AF350
Alexa Fluor 405	NBP2-03952AF405
Alexa Fluor 488	NBP2-03952AF488
Alexa Fluor 532	NBP2-03952AF532
Alexa Fluor 594	NBP2-03952AF594
Alexa Fluor 647	NBP2-03952AF647
Alexa Fluor 700	NBP2-03952AF700
Alexa Fluor 750	NBP2-03952AF750
Alexa Fluor Plus 405	NBP2-03952AFP405
Alexa Fluor Plus 488	NBP2-03952AFP488
Alexa Fluor Plus 555	NBP2-03952AFP555
Alexa Fluor Plus 594	NBP2-03952AFP594
Alexa Fluor Plus 647	NBP2-03952AFP647
Alexa Fluor Plus 680	NBP2-03952AFP680
Allophycocyanin	NBP2-03952APC
Biotin	NBP2-03952B
CoraFluor 1	NBP2-03952CL1
DyLight 350	NBP2-03952UV
DyLight 405	NBP2-03952V
DyLight 488	NBP2-03952G
DyLight 550	NBP2-03952R
DyLight 594	NBP2-03952DL594
DyLight 650	NBP2-03952C
DyLight 680	NBP2-03952FR
DyLight 755	NBP2-03952IR
FITC	NBP2-03952F
HRP	NBP2-03952H
Janelia Fluor 525	NBP2-03952JF525
Janelia Fluor 549	NBP2-03952JF549
Janelia Fluor 646	NBP2-03952JF646
Janelia Fluor 669	NBP2-03952JF669
PE	NBP2-03952PE
PerCP	NBP2-03952PCP
mFluor Violet 450 SE	NBP2-03952MFV450
mFluor Violet 500 SE	NBP2-03952MFV500
mFluor Violet 610 SE	NBP2-03952MFV610

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NBP2-03955

Podoplanin Antibody (pmab-1) - BSA Free

(2 Publications)

0.1 mg

NBP2-03955

Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-03955 and APC-conjugated secondary antibody were used for this test.

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-6743A

Alt Catalog# IMG-6743A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-03955AF350
Alexa Fluor 405	NBP2-03955AF405
Alexa Fluor 488	NBP2-03955AF488
Alexa Fluor 532	NBP2-03955AF532
Alexa Fluor 594	NBP2-03955AF594
Alexa Fluor 647	NBP2-03955AF647
Alexa Fluor 700	NBP2-03955AF700
Alexa Fluor 750	NBP2-03955AF750
Alexa Fluor Plus 405	NBP2-03955AFP405
Alexa Fluor Plus 488	NBP2-03955AFP488
Alexa Fluor Plus 555	NBP2-03955AFP555
Alexa Fluor Plus 594	NBP2-03955AFP594
Alexa Fluor Plus 647	NBP2-03955AFP647
Alexa Fluor Plus 680	NBP2-03955AFP680
Allophycocyanin	NBP2-03955APC
Biotin	NBP2-03955B
CoraFluor 1	NBP2-03955CL1
DyLight 350	NBP2-03955UV
DyLight 405	NBP2-03955V
DyLight 488	NBP2-03955G
DyLight 550	NBP2-03955R
DyLight 594	NBP2-03955DL594
DyLight 650	NBP2-03955C
DyLight 680	NBP2-03955FR
DyLight 755	NBP2-03955IR
FITC	NBP2-03955F
HRP	NBP2-03955H
Janelia Fluor 525	NBP2-03955JF525
Janelia Fluor 549	NBP2-03955JF549
Janelia Fluor 646	NBP2-03955JF646
Janelia Fluor 669	NBP2-03955JF669
PE	NBP2-03955PE
PerCP	NBP2-03955PCP
mFluor Violet 450 SE	NBP2-03955MFV450
mFluor Violet 500 SE	NBP2-03955MFV500
mFluor Violet 610 SE	NBP2-03955MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-03955SS
Azide and BSA Free	NBP2-80917

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NBP1-90211

Podoplanin Antibody - BSA Free

(6 Publications)

0.1 ml

NBP1-90211

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human endometrium shows moderate membranous positivity in endothelium of lymphatic vessels.

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human rectum shows moderate membranous positivity in endothelium of lymphatic vessels.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90211-25ul

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NB600-1013

Podoplanin Antibody (18H5) - Azide and BSA Free

(2 Publications)

0.1 mg

NB600-1013

Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.

Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Flow analysis of Podoplanin expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). As expected there is no expression of Podoplanin detectable in the HUVECs but a strong one in the HDMVEC.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1013AF350
Alexa Fluor 405	NB600-1013AF405
Alexa Fluor 488	NB600-1013AF488
Alexa Fluor 532	NB600-1013AF532
Alexa Fluor 594	NB600-1013AF594
Alexa Fluor 647	NB600-1013AF647
Alexa Fluor 700	NB600-1013AF700
Alexa Fluor 750	NB600-1013AF750
Alexa Fluor Plus 405	NB600-1013AFP405
Alexa Fluor Plus 488	NB600-1013AFP488
Alexa Fluor Plus 555	NB600-1013AFP555
Alexa Fluor Plus 594	NB600-1013AFP594
Alexa Fluor Plus 647	NB600-1013AFP647
Alexa Fluor Plus 680	NB600-1013AFP680
Allophycocyanin	NB600-1013APC
Biotin	NBP1-18600
CoraFluor 1	NB600-1013CL1
DyLight 350	NB600-1013UV
DyLight 405	NB600-1013V
DyLight 488	NB600-1013G
DyLight 550	NB600-1013R
DyLight 594	NB600-1013DL594
DyLight 650	NB600-1013C
DyLight 680	NB600-1013FR
DyLight 755	NB600-1013IR
FITC	NB600-1013F
HRP	NB600-1013H
Janelia Fluor 525	NB600-1013JF525
Janelia Fluor 549	NB600-1013JF549
Janelia Fluor 646	NB600-1013JF646
Janelia Fluor 669	NB600-1013JF669
PE	NB600-1013PE
PerCP	NB600-1013PCP
mFluor Violet 450 SE	NB600-1013MFV450
mFluor Violet 500 SE	NB600-1013MFV500
mFluor Violet 610 SE	NB600-1013MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1013-0.02mg

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NBP3-11971

Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free

0.2 mg

NBP3-11971

Immunocytochemistry /Immunofluorescence: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP3-11971 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-11971 at 0.01 ug/ml, 0.1 ug/ml, 0.001 ug/ml and 0.003 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11971AF350
Alexa Fluor 405	NBP3-11971AF405
Alexa Fluor 488	NBP3-11971AF488
Alexa Fluor 532	NBP3-11971AF532
Alexa Fluor 594	NBP3-11971AF594
Alexa Fluor 647	NBP3-11971AF647
Alexa Fluor 700	NBP3-11971AF700
Alexa Fluor 750	NBP3-11971AF750
Alexa Fluor Plus 405	NBP3-11971AFP405
Alexa Fluor Plus 488	NBP3-11971AFP488
Alexa Fluor Plus 555	NBP3-11971AFP555
Alexa Fluor Plus 594	NBP3-11971AFP594
Alexa Fluor Plus 647	NBP3-11971AFP647
Alexa Fluor Plus 680	NBP3-11971AFP680
Allophycocyanin	NBP3-11971APC
Biotin	NBP3-11971B
CoraFluor 1	NBP3-11971CL1
DyLight 350	NBP3-11971UV
DyLight 405	NBP3-11971V
DyLight 488	NBP3-11971G
DyLight 550	NBP3-11971R
DyLight 594	NBP3-11971DL594
DyLight 650	NBP3-11971C
DyLight 680	NBP3-11971FR
DyLight 755	NBP3-11971IR
FITC	NBP3-11971F
HRP	NBP3-11971H
Janelia Fluor 525	NBP3-11971JF525
Janelia Fluor 549	NBP3-11971JF549
Janelia Fluor 646	NBP3-11971JF646
Janelia Fluor 669	NBP3-11971JF669
PE	NBP3-11971PE
PerCP	NBP3-11971PCP
mFluor Violet 450 SE	NBP3-11971MFV450
mFluor Violet 500 SE	NBP3-11971MFV500
mFluor Violet 610 SE	NBP3-11971MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-11971-0.025mg

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MAB11608

Podoplanin Antibody (1090106) [Unconjugated]

100 μg

MAB11608

HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11608) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11608) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of podocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of podocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of alveoli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of alveoli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11608U-100UG
Alexa Fluor 405	FAB11608V-100UG
Alexa Fluor 488	FAB11608G-100UG
Alexa Fluor 532	FAB11608X-100UG
Alexa Fluor 594	FAB11608T-100UG
Alexa Fluor 647	FAB11608R-100UG
Alexa Fluor 700	FAB11608N-100UG
Alexa Fluor 750	FAB11608S-100UG
Alexa Fluor Plus 405	FAB11608AFP405-100UG
Alexa Fluor Plus 488	FAB11608AFP488-100UG
Alexa Fluor Plus 555	FAB11608AFP555-100UG
Alexa Fluor Plus 594	FAB11608AFP594-100UG
Alexa Fluor Plus 647	FAB11608AFP647-100UG
Alexa Fluor Plus 680	FAB11608AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11608-SP

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NBP2-36058

Podoplanin Antibody (RTD4E10) - Azide and BSA Free

0.1 mg

NBP2-36058

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

, , ,

Applications: , , ,

Host:

Hamster Monoclonal

Host: Hamster Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36058AF350
Alexa Fluor 405	NBP2-36058AF405
Alexa Fluor 488	NBP2-36058AF488
Alexa Fluor 532	NBP2-36058AF532
Alexa Fluor 594	NBP2-36058AF594
Alexa Fluor 647	NBP2-36058AF647
Alexa Fluor 700	NBP2-36058AF700
Alexa Fluor 750	NBP2-36058AF750
Alexa Fluor Plus 405	NBP2-36058AFP405
Alexa Fluor Plus 488	NBP2-36058AFP488
Alexa Fluor Plus 555	NBP2-36058AFP555
Alexa Fluor Plus 594	NBP2-36058AFP594
Alexa Fluor Plus 647	NBP2-36058AFP647
Alexa Fluor Plus 680	NBP2-36058AFP680
Allophycocyanin	NBP2-36058APC
Biotin	NBP1-18603
CoraFluor 1	NBP2-36058CL1
DyLight 350	NBP2-36058UV
DyLight 405	NBP2-36058V
DyLight 488	NBP2-36058G
DyLight 550	NBP2-36058R
DyLight 594	NBP2-36058DL594
DyLight 650	NBP2-36058C
DyLight 680	NBP2-36058FR
DyLight 755	NBP2-36058IR
FITC	NBP2-36058F
HRP	NBP2-36058H
Janelia Fluor 525	NBP2-36058JF525
Janelia Fluor 549	NBP2-36058JF549
Janelia Fluor 646	NBP2-36058JF646
Janelia Fluor 669	NBP2-36058JF669
PE	NBP2-36058PE
PerCP	NBP2-36058PCP
mFluor Violet 450 SE	NBP2-36058MFV450
mFluor Violet 500 SE	NBP2-36058MFV500
mFluor Violet 610 SE	NBP2-36058MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-36058-0.02mg

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NBP2-89552

Podoplanin Antibody (066) - Azide and BSA Free

50 ul

NBP2-89552

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89552AF350
Alexa Fluor 405	NBP2-89552AF405
Alexa Fluor 488	NBP2-89552AF488
Alexa Fluor 532	NBP2-89552AF532
Alexa Fluor 594	NBP2-89552AF594
Alexa Fluor 647	NBP2-89552AF647
Alexa Fluor 700	NBP2-89552AF700
Alexa Fluor 750	NBP2-89552AF750
Alexa Fluor Plus 488	NBP2-89552AFP488
Alexa Fluor Plus 555	NBP2-89552AFP555
Alexa Fluor Plus 594	NBP2-89552AFP594
Alexa Fluor Plus 647	NBP2-89552AFP647
Alexa Fluor Plus 680	NBP2-89552AFP680
Allophycocyanin	NBP2-89552APC
Biotin	NBP2-89552B
CoraFluor 1	NBP2-89552CL1
DyLight 350	NBP2-89552UV
DyLight 405	NBP2-89552V
DyLight 488	NBP2-89552G
DyLight 550	NBP2-89552R
DyLight 594	NBP2-89552DL594
DyLight 650	NBP2-89552C
DyLight 680	NBP2-89552FR
DyLight 755	NBP2-89552IR
FITC	NBP2-89552F
HRP	NBP2-89552H
Janelia Fluor 525	NBP2-89552JF525
Janelia Fluor 549	NBP2-89552JF549
Janelia Fluor 646	NBP2-89552JF646
Janelia Fluor 669	NBP2-89552JF669
PE	NBP2-89552PE
PerCP	NBP2-89552PCP
mFluor Violet 450 SE	NBP2-89552MFV450
mFluor Violet 500 SE	NBP2-89552MFV500
mFluor Violet 610 SE	NBP2-89552MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89552-100ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Podoplanin Antibodies

Podoplanin Antibody [Unconjugated]

Podoplanin Antibody (8.1.1) - BSA Free

Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Podoplanin Antibody [Unconjugated]

Podoplanin Antibody (NZ-1.2) - BSA Free

Podoplanin Antibody (pmab-1) - BSA Free

Podoplanin Antibody - BSA Free

Podoplanin Antibody (18H5) - Azide and BSA Free

Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free

Podoplanin Antibody (1090106) [Unconjugated]

Podoplanin Antibody (RTD4E10) - Azide and BSA Free

Podoplanin Antibody (066) - Azide and BSA Free