CLEC-2/CLEC-1B Antibody [Unconjugated]

Images

 
Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by ...read more
Western blot shows lysates of mouse kidney tissue, rat kidney tissue, and mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CLEC-2/CLEC-1B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-2/CLEC1B
Gln58-Pro229
Accession # AAF36777
Specificity
Detects human, mouse, and rat CLEC-2/CLEC1B in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CLEC-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLEC1B
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1718 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-2/CLEC-1B Antibody [Unconjugated]

  • 1810061I13Rik
  • CLEC1B
  • CLEC2
  • CLEC-2
  • CLEC2B
  • CLEC2PRO1384
  • C-type lectin domain family 1 member B
  • C-type lectin domain family 1, member B
  • C-type lectin-like receptor 2
  • C-type lectin-like receptor-2
  • QDED721

Background

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).
  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLEC-2/CLEC-1B Antibody (AF1718)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Functional Assay, ICC, IP, WB.


Filter By Application
Functional Assay
(1)
ICC
(1)
IP
(1)
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(2)
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(4)
Mouse
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Showing Publications 1 - 5 of 5.
Publications using AF1718 Applications Species
A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice J Am Heart Assoc, 2017;6(9):. 2017 [PMID: 28939717] (WB,, Mouse) WB Mouse
C Delierneux, N Donis, L Servais, O Wéra, C Lecut, M Vandereyke, L Musumeci, S Rahmouni, J Schneider, JA Eble, P Lancellott, C Oury Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides J. Thromb. Haemost, 2017;0(0):. 2017 [PMID: 28296036] (IP,, Human) IP Human
Del Rey M, Fare R, Izquierdo E, Usategui A, Rodriguez-Fernandez J, Suarez-Fueyo A, Canete J, Pablos J Clinicopathological correlations of podoplanin (gp38) expression in rheumatoid synovium and its potential contribution to fibroblast platelet crosstalk. PLoS ONE, 2014;9(6):e99607. 2014 [PMID: 24932813] (WB,, Human) WB Human
Fuller GL, Williams JA, Tomlinson MG, Eble JA, Hanna SL, Pohlmann S, Suzuki-Inoue K, Ozaki Y, Watson SP, Pearce AC The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade. J. Biol. Chem., 2007;282(17):12397-409. 2007 [PMID: 17339324] (Functional Assay,, Human) Functional Assay Human
Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. J. Virol., 2006;80(18):8951-60. 2006 [PMID: 16940507] (ICC,, Human) ICC Human

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Bioinformatics

Gene Symbol CLEC1B
Entrez
Uniprot