PML Protein Antibody - BSA Free

Western Blot: PML Protein Antibody [NBP2-94515] - Analysis of extracts of various cell lines, using PML Protein . Exposure time: 3min.

Immunohistochemistry: PML Protein Antibody - BSA Free [PML Protein] - Immunohistochemistry analysis of paraffin-embedded human appendix using PML Protein Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PML Protein Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 571-611 of human PML Protein (NP_150241.2). SSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PML
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PML Protein Antibody - BSA Free

• MYLPP8675
• Promyelocytic leukemia protein
• promyelocytic leukemia
• RING finger protein 71
• RNF71probable transcription factor PML
• TRIM19promyelocytic leukemia, inducer of
• tripartite motif protein TRIM19
• Tripartite motif-containing protein 19

Background

PML Protein is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PML