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SP100 Antibody

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Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Orthogonal Strategies: Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows moderate nuclear speckle positivity.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human placenta shows moderate nuclear speckle positivity.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human tonsil shows moderate nuclear speckle positivity.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining in human tonsil and cerebral cortex tissues.. Corresponding SP100 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human colon.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human testis.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human spleen.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to ...read more
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, Micro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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SP100 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSE
Specificity
Specificity of human SP100 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SP100
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Micro
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Micro reported in scientific literature (PMID 28095508).
Control
SP100 Knockout HeLa Cell Lysate
Control Peptide
SP100 Protein (NBP1-86060PEP)
Publications
Read Publications using
NBP1-86060 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24194542).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SP100 Antibody

  • DKFZp686E07254
  • FLJ00340
  • FLJ34579
  • lysp100b
  • nuclear antigen Sp100
  • nuclear autoantigen Sp-100
  • Nuclear dot-associated Sp100 protein
  • SP100 nuclear antigen
  • SP100-HMG nuclear autoantigen
  • Speckled 100 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
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Western Blot: NUP210 Antibody [NBP2-93742] - Analysis of extracts of various cell lines, using NUP210 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
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Immunocytochemistry/Immunofluorescence: NUP210 Antibody [NBP2-93742] - Analysis of L929 cells using NUP210 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Publications for SP100 Antibody (NBP1-86060)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Micro.


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Control Lysate(s)

SP100 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SP100