PML Protein Antibody - BSA Free

Western Blot: PML Protein Antibody [NBP2-19872] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunoprecipitation: PML Protein Antibody [NBP2-19872] - Immunoprecipitation of PML protein from A549 whole cell extracts using 5 ug of PML antibody [N1N2], N-term. Western blot analysis was performed using PML antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Pm, RM
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PML Protein Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PML Protein Antibody - BSA Free (NBP2-19872) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PML Protein. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (98%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PML
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PML Protein Antibody - BSA Free

• MYLPP8675
• Promyelocytic leukemia protein
• promyelocytic leukemia
• RING finger protein 71
• RNF71probable transcription factor PML
• TRIM19promyelocytic leukemia, inducer of
• tripartite motif protein TRIM19
• Tripartite motif-containing protein 19

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PML
• Uniprot:
  • P29590(Mouse)